Past Annual Meetings:
10th Annual Meeting, Ottawa, Ontario
September 16 - 19,
2005
Presentation Abstracts | Poster
Abstracts | Photos |Abstract Book (pdf format) | Organizing Committee
Presentation Abstract Index:
The following are the titles of papers presented at the 10th annual meeting organized in alphabetical order by first author's last name. Links will take you to the abstract.
Evaluation of Eastern Racer (Coluber constrictor) habitat use and replacement: a cooperative effort of the Vermont Departments of
Transportation and Fish and Wildlife. Jim Andrews
Introduced American bullfrog (Rana catesbeiana) removal in the South Okanagan. Sara Ashpole, David C. Cunnington, Ryan Noble
A comparison of preferred and optimal temperatures in the common map turtle (Graptemys geographica). Elad Ben-Ezra, Gregory Bulté, Gabriel
Blouin-DemersWhat is critical habitat? The case of the Jefferson salamander. Karine Beriault, J.P. Bogart
PLENARY ADDRESS : Thermoregulation, habitat use, and fitness in reptiles. Gabriel Blouin-Demers
Investigation of bullfrogs in sub-watersheds of the Yamaska River: Health status of bullfrogs. Monique Boily, Philip Spear, Anicha Nkoua, Sylvia
Ruby, Catherine Dimacacos, Michel Fournier, Harrie Salo
Investigation of bullfrogs in sub-watersheds of the Yamaska River: General introduction and water quality. Monique Boily, Philip Spear*,
Guillaume Cardin, Daniel Rivest, Phillipe Juneau, Christian DeBlois, Nathalie Dassylva, Isabelle Giroux, Denis Laliberté, Denis
Forestry guidelines to conserve rare amphibians and aeptiles: A case study from Massachusetts. Leslie Bol, Henry Woolsey
Threats and limiting factors to nesting and embryo hatch success of the spiny softshell. Ryan Bolton, Ronald J. Brooks
Accounting for variability in a population viability analysis of Nova Scotia's Blanding's turtle (Emydoidea blandingii). Guillaume Bourque, Tom
Herman, J.A. McNeil, D.D. Hurlburt
Rattlesnake conservation in the south Okanagan Valley. J. Brown, C.A. Bishop*, B. Baptiste, M. Sarell, S. Austen, M. Holm
Identifying habitat features at local and landscape scales that affect the distribution and abundance of anuran amphibians in the western boreal
forest. Constance Browne, Shelley Boss, A. Lee Foote, Cynthia A Paszkowski*
Quantifying age and sex specific zebra mussels predation by the common map turtle (Graptemys geographica) using stable carbon isotopes:
Preliminary results. Gregory Bulté, Gabriel Blouin-Demers
Effects of commercial fishing traps on a map turtle (Graptemys geographica) population in Thompson's Bay, St-Lawrence River. Marie-Andree
Carrière, Gabriel Blouin-Demers
Linking science and stewardship through public education with the Nova Scotia Blanding's turtle (Emydoidea blandingii). Brennan Caverhill and
Tom Herman
Ontario's conservation responsibility for reptiles: the development of a conservation responsibility index based on proportion of range and current
conservation ranks. Susan Cowin, Michael Oldham, Joe Cebek
Consequences of early UV-B exposure on amphibian development and metamorphosis. Maxine Croteau, David Lean, Vance Trudeau
Are Ontario reptiles on the road to extinction? Anthropogenic disturbance and reptile distributions within the province. Joe Crowley and Ronald J.
Brooks
Characteristics of turtle populations in small ponds along roads. Jean-François Desroches, Isabelle Picard
Looking for nests to find the rare four-toed salamander (Hemidactylium scutatum): Technical approach and nest characterisation. Jean-François
Desroches, Daniel Pouliot*
Interactive effects of malathion exposure and ranaviral infections in wood frogs (Rana sylvatica). Amanda Duffus, Craig Brunetti, Bruce Pauli,
Michael Berrill
The effect of the configuration of habitat relative to roads on pond-dwelling amphibians. Felix Eigenbrod, S.J. Hecnar, L. Fahrig
The relative effects of forested, agricultural and urban landscapes on amphibian communities in eastern Ontario. Sara Gagné, Lenore Fahrig
Assessing prevalence of chytrid fungus (Batrachochytrium dendrobatidis) innative amphibians and bullfrogs (Rana catesbeiana) on Vancouver
Island, British Columbia. Purnima Govindarajulu, Trenton Garner, Bradley Anholt
Effects of sexual size dimorphism on diet specialization in the common map turtle (Graptemys geographica). Marie-Ange Gravel, Gregory Bulté,
Gabriel Blouin-Demers
Paved roads as barriers to amphibian movements. Mireille Gravel, Marc Mazerolle, Marc-André Villard
Thermal ecology of overwintering wood turtles (Glyptemys insculpta) at the species' northern range limit. William Greaves, Jacqueline Litzgus
The Fowler's toad recovery plan. David Green, Anne Yagi
Demographic traits of introduced common wall lizards (Podarcis muralis) on Vancouver Island. Patrick Gregory
Exploring the limits of a range: Thamnophis sauritus in Nova Scotia. T. Herman, R. Wassersug, J. McNeil, J. Todd, S. Bell, G. Bourque, B.
Caverhill,M. Lawton, E. Newton, N. Seguin, J. Caron
Xenopus tropicalis: a novel surrogate species for amphibian toxicology. Natacha Hogan, Vance Trudeau
The genetics of peripheral populations. Briar Howes, Stephen Lougheed
The effect of sex ratio on sexual selection in painted turtles (Chrysemys picta). Elinor Hughes, Ronald J. Brooks
Large body temperature fluctuations of eastern foxsnakes (Elaphe gloydi) duringvoluntary cold-water swimming in their natural habitats. Anna
Lawson, Carrie A. MacKinnon, E.D. Stevens, R.J. Brooks
Amphibian conservation: Back to the future. David Lesbarrères, Mike Fowler
Survivorship and differential longevity in the spotted turtle (Clemmys guttata). Jacqueline Litzgus
PLENARY ADDRESS : Peripheral populations and their potential conservation value. Stephen Lougheed
Why did the reptile cross the road? Landscape factors associated with road mortality of snakes and turtles in the Southeastern Georgian Bay
area. Carrie A. MacKinnon, Lisa Moore, Ronald J. Brooks
Phylogeography of stream salamanders in Quebec and Labrador. Tricia M. Markle, David Green
A valuable tool to assess the strength of biological hypotheses in herpetology: Akaike's Information Criterion (AIC). Marc Mazerolle
Impacts of row crop agriculture on sexual development of anurans. Tana McDaniel, Pamela Martin, Chris Marvin, Mark McMaster, Jim Sherry
Update from the Kawartha Turtle Trauma Centre. Kristy McNab
Genetic structure of the Eastern Red-backed salamander (Plethodon cinereus) in an urban landscape. Sarah Noël, Martin Ouellet, Patrick
Galois, Françcois-Joseph Lapointe
The drastic decline of the Western Chorus Frog (Pseudacris triseriata) in southwestern Québec. Isabelle Picard, Jean-François Desroches
How far from the nesting site should we protect the four-toed salamander (Hemidactylium scutatum)? A case of urban conservation. Daniel
Pouliot, Héloïse Bastien
Effect of temperature on physiology and behaviour in two colour morphs of the red-backed salamander (Plethodon cinereus). Daniel Reeves,
Jacqueline Litzgus, David Hackett
Annual temperature variations affect clutch frequency and egg size in a northern population of painted turtles (Chrysemys picta). Njal Rollinson,
Ronald J. Brooks
Wood Turtle (Clemmys insculpta) habitat requirements and movements in New Brunswick. Vanessa Roy, Graham Forbes
Factors affecting amphibian distribution and community structure in Nova Scotia. Ronald Russell
Beaver (Castor canadensis) as a surrogate species for conserving anuran amphibians on boreal streams. Cameron Stevens, Cynthia
Paszkowski, A. Lee Foote
Local and regional scale habitat selection by wood turtles (Glyptemys insculpta) at the northern limit of their range. Pamela Wesley, Ronald J.
Brooks
Does forest harvesting create or destroy aquatic amphibian habitat? Elke Wind
Poster Abstract Index:
The following are the titles of posters presented at the 10th annual meeting organized in alphabetical order by first author's last name. Links will take you to the abstract.
Genetic Differentiation and conservation of Wood Turtle (Glyptemys insculpta) populations throughout their range. Marina Amato, Ronald J.
Brooks, Jinzhong Fu
Development of integrated indicators for monitoring the biodiversity of the St. Lawrence wetlands. Alain Armellin, Martin Jean, Caroline Savage,
Magella Pelletier
Pesticide exposure and reproductive effects in native amphibian species using agricultural habitat, South Okanagan, British Columbia (2003-2005). Sara Ashpole, Christine A. Bishop, John Elliott
Can the timing and location of wetland habitat enhancement increase the success of the Northern Leopard Frog Recovery Project on the Creston
Valley Wildlife Management Area in southeastern British Columbia? Marc-André Beaucher, Doug Adama
Age estimation in the bullfrog using skeletochronology. Marie-Lou Breton, Philip Spear, Marc Levasseur, Monique Boily*
Amphibian diseases in Ontario: Chytridiomycosis and ranaviral Disease. Michelle Charbonneau, Christina Fridgen, Michael Berrill, Bruce Pauli
Effects of environmentally relevant concentrations of atrazine on gonadal development of snapping turtles (Chelydra serpentina). Shane de Solla,
Pamela Martin, Kimberly Fernie, Brad Park, Greg Mayne
Comparison of turtle species abundance and richness in damaged and recovered lakes in Sudbury, Ontario. Crystal Demmer, Tonia Van
Kempen, Jacqueline Litzgus
Testicular degeneration in adult male bullfrogs (R. catesbeiana) from sampling sites in the Yamaska River basin. Catherine Dimacacos, Sylvia
Ruby, Pamela Giancola, Monique Boily, Philip Spear, Michel Fournier
Toxicological field studies of sexual differentiation and reproduction in R. catesbeiana tadpoles collected from sampling sites in the Yamaska river
basin. Catherine Dimacacos, Sylvia Ruby, Pamela Giancola, Monique Boily, Philip Spear, Michel Fournier
Early exposure to 17�-ethinylestradiol alters sex ratios and gonadal morphology of developing leopard frogs (Rana pipiens). Paula Duarte,
Natacha Hogan, Bruce Pauli, Michael Wade, David Lean, Vance Trudeau
Behavioural thermoregulation in wood turtles, chasing the sun slowly! Yohann Dubois, Donald Thomas, Bill Shipley
Quick identification of Ambystoma hybrids from the Jefferson salamander complex. Jeanne Dumoulin, Sarah Noël, Martin Ouellet, Patrick Galois,
François-Joseph Lapointe
Impacts of pond connectivity on amphibian life stages at Delta Marsh, Manitoba. Katarzyna Dyszy, Dale Wrubleski, John Spence
Demography and behaviour of a Georgian Bay population of the spotted turtle (Clemmys guttata). Jean Enneson, Jacqueline Litzgus
Landscape and local factors associated with vertebrate roadkill in southern Ontario. Bob Farmer, Ronald J. Brooks
Assessing DNA damage in frogs from agricultural areas of southwestern Ontario. Robyn Ferguson, Bruce Pauli,* Pamela Martin, Tana McDaniel,
Chris Marvin, Loren Knopper
Chronic effects of atrazine herbicide on the development of Northern Leopard Frog (Rana pipiens) tadpoles. Christina Fridgen, Bruce Pauli,
Michael Berrill, Ken Doe, Paula Jackman
Population dynamics of the wood turtle in the greater Kouchibouguac ecosystem, New Brunswick/Dynamique des populations de tortues des bois
dans le grand écosystème de Kouchibouguac, Nouveau-Brunswick. Mireille Gravel, Éric Tremblay2, Tom Herman3, and Donald McAlpine4
Influence of water conditions on the embryonic survivorship of the Oregon spotted frog (Rana pretiosa). René McKibbin, Christine A. Bishop,
Russ Haycock
Excavation of freshwater turtle eggs is a non-deleterious method for obtaining fecundity and egg morphometric data. Jason Samson, Elinor
Hughes*, Ronald J, Brooks
Emergent infectious diseases in the Leopard Frog (Rana pipiens) in central Ontario. Valerie St-Amour, Michael Berrill
Status of the Western Toad and its use of 'borrow pits' in the foothills of west-central Alberta. Cameron Stevens, Cynthia Paszkowski, David
Stringer, Shelly Boss
Does multiple paternity increase with female size in the Common Map Turtle (Graptemys geographica)?: A work in progress. Carine Verly,
Gregory Bulté, Gabriel Blouin-Demers
Conference Abstracts
GENETIC DIFFERENTIATION AND CONSERVATION OF WOOD TURTLE (Glyptemys insculpta) POPULATIONS THROUGHOUT THEIR
RANGE
Marina Amato, Ron J. Brooks, Jinzhong Fu
Department of Integrative Biology, University of Guelph, Guelph, Ontario N1G 2W1, Tel: (519) 824-4120, Fax: (519) 767-1656,
[email protected] , [email protected] , [email protected]
The wood turtle (Glyptemys insculpta) is endemic to North America and ranges from southern Ontario and Quebec, east to New Brunswick and
Nova Scotia, west to Minnesota and south to Virginia. However, this range is discontinuous and wood turtle populations within it are generally
isolated. Most wood turtle populations in Canada and the United States are declining, and the few populations reported as stable are in areas
with little human activity or access. The purpose of this research is to document patterns of genetic differentiation among wood turtle populations
throughout their range. Mitochondrial DNA sequence data will be used to provide insight on the genetic structure, phylogeography, postglacial
dispersal and conservation of G. insculpta in North America. Specifically, this study will aim to: (i) assess species-wide phylogeographical
structure, (ii) trace the potential path wood turtles took during postglacial re-colonization, (iii) understand the taxonomic status and assist in
determining conservation priorities for wood turtle populations throughout their range.
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EVALUATION OF EASTERN RACER (Coluber constrictor) HABITAT USE AND REPLACEMENT: A COOPERATIVE EFFORT OF THE
VERMONT DEPARTMENTS OF TRANSPORTATION AND FISH AND WILDLIFE
James S. Andrews
642 Smead Road, Salisbury, VT 05753 & The Vermont Reptile and Amphibian Atlas Project, Middlebury College, Middlebury VT 05753, 802-443-5648, 802-443-2072 FAX, [email protected]
During fieldwork for the Vermont Reptile and Amphibian Atlas Project, a population of Eastern Racers was discovered in southern Vermont. This
was the first documented report of the species in the state since 1985. As a result of this discovery, the species is now listed as threatened by
the state of Vermont. Unfortunately, the Vermont Department of Transportation has plans to construct a truck-weighing station on the discovery
site. As a result, the Departments of Transportation and Fish and Wildlife are taking a unique proactive and cooperative approach in advance of
the permitting process. A study funded by the Department of Transportation (VTrans) was undertaken to determine the function and importance
of the proposed development site for the Eastern Racer population. Results of the study are being used to design replacement habitat to be
constructed prior to the beginning of the development. Radio tracking of two adult male snakes revealed the location of the den and facilitated
the implantation of passive integrated transponders (PIT tags) into seven adult racers. In addition, the studies indicate that the site is peripheral
to the core of activity but important as a foraging area and as a connecting corridor to appropriate habitat further south. Radio tracking also
revealed that the population only utilizes open early-successional habitat created by the margins of a busy interstate and a power line cut.
Consequently, adjacent state lands will be cleared to provide replacement foraging habitat and a connecting corridor of open land around the
development site.
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DÉVELOPPEMENT D'INDICATEURS INTÉGRÉS POUR LE SUIVI DE LA BIODIVERSITÉ DES MILIEUX HUMIDES DU SAINT-LAURENT/DEVELOPMENT OF INTEGRATED INDICATORS FOR MONITORING THE BIODIVERSITY OF THE ST. LAWRENCE WETALNDS
Alain Armellin, Martin Jean, Caroline Savage and Magella Pelletier
St. Lawrence Centre, State of St. Lawrence, Environment Canada, Quebec Region. Telephone: (514) 283-7000, Fax: (514) 496-1930, E-mail
address: [email protected] ; [email protected] ; [email protected] ; [email protected]
Les milieux humides sont considérés comme des éléments essentiels d'un écosystème fluvial. En plus de leurs nombreuses fonctions, les
milieux humides contribuent grandement à la diversité tant végétale qu'animale. La végétation des milieux humides offre des habitats de qualité à
la faune. Selon l'approche écosystémique, les indicateurs de la biodiversité des milieux humides doivent intégrer les informations sur la
répartition spatiale des communautés végétales et sur l'abondance et la diversité de la faune, incluant autant l'herpétofaune que les
macroinvertébrés dans différents paysages fluviaux. Un projet-pilote, visant à évaluer la flore et la faune à différentes échelles spatiales durant la
même période, a été amorcé au lac Saint-Pierre en 2004. Pour ce projet, nous avons retenu l'analyse des communautés végétales des milieux
humides et des assemblages d'anoures qui leurs sont associés, car les anoures jouent un rôle crucial dans les milieux humides à cause de leur
position trophique et de leur forte biomasse.
Wetlands are considered as essential elements of a river ecosystem. In addition to their many functions, wetlands largely contribute to plant and
animal diversity. Wetland vegetation supplies high-quality habitats to wildlife. According to the ecosytemic approach, indicators of wetland
biodiversity should integrate information on spatial distribution of plant communities and on abundance and diversity of wildlife, including as much
herpetofauna as macroinvertebrates, in various fluvial landscapes. A pilot project on the assessment of plants and wildlife on various spatial
scales during the same period was initiated in Lake Saint-Pierre in 2004. For this project, we have chosen to analyse plant communities and the
associated assemblages of anurans, because these animals are crucial to wetlands, due to their trophic level and their large biomass.
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INTRODUCED AMERICAN BULLFROG (Rana catesbeiana) REMOVAL IN THE SOUTH OKANAGAN
Sara L. Ashpole1, Dave C. Cunnington1, Ryan Noble2
1Canadian Wildlife Service. 5421 Robertson Rd. Delta, British Columbia, V4K 3N2, Canada,
2British Columbia Conservation Corps, [email protected] ; [email protected]
In the past few years, invasive American Bullfrogs (Rana catesbeiana) have been detected at four locations in the South Okanagan. These
populations of bullfrogs pose a great hazard to native amphibians at risk, including the COSEWIC listed Spadefoot, Tiger salamander, and
Western toad. It is believed that these frogs are a remnant population originating from the food industry in the 1950s. In 2004 and 2005 physical
methods of removal have included: dip-netting, hand capture, seining, electro-shocking, and modified traps. The total number of individuals
removed to date, and their life stage included: 88 adults; 32 juveniles; 11,222 tadpoles; and 20 egg masses. To contain the populations and
reduce the probability of bullfrog migration, a semi-permanent exclusion fence has been constructed around two infected permanent ponds. Even
though, removal methods have proven successful at suppressing the adult and reproductive success of the populations, complete eradication will
require continued effort and potentially more drastic measures taken. Intense surveying in the South Okanagan has not detected bullfrogs at any
additional ponds. The proximity of these ponds to each other is less than a few hundred meters, with the closest pond only 300m from Lake
Osoyoos and the Okanagan River system. Anecdotal accounts near Lake Osoyoos raises great concern that this species may have a much
wider local distribution than currently reported.
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PESTICIDE EXPOSURE AND REPRODUCTIVE EFFECTS IN NATIVE AMPHIBIAN SPECIES USING AGRICULTURAL HABITAT, SOUTH
OKANAGAN, BRITISH COLUMBIA (2003-2005)
Sara L. Ashpole, Christine A. Bishop, John Elliott
Canadian Wildlife Service. 5421 Robertson Rd. Delta, British Columbia, V4K 3N2, Canada. [email protected]; [email protected];
[email protected]
The Okanagan valley in BC is an area of intensive agriculture where 80% of the natural wetlands and riparian zones have been drained or
altered. In total, 64 ponds, including 23 agricultural ponds, were surveyed to determine adult breeding, larval productivity, and relative population
densities (2003 - 2005). To assess the risk of amphibian populations to multiple stressor effects of pesticides, we conducted two in situ
experiments focusing on early native amphibian stages of development. Hatching success, tadpole survival, and abnormalities were recorded.
Enclosures with eggs were placed in ponds located in either conventional orchards subjected to pesticide applications (azinphos-methyl, carbaryl,
diazinon, endosulfan, pirimicarb), or in organic orchards, or non-agricultural control ponds. Water samples were collected for pesticide analyses
at standard times and after known spray events. Historic contaminant levels in sediments were at relatively low to non-detectable levels, with the
exception of DDT and its metabolites (DDT 0.24 - 47 ng/g d.w. (dry weight); DDE 2.52 - 1938.9 ng/g d.w.; DDD 5.26-1334.4 ng/g d.w.). In 2004,
Spadefoot (Spea intermontana) and Western Toad (Bufo boreas) eggs were placed in conventional (N=2) and organic orchards (N=3). In 2004,
substantial mortality was observed in both species at one of our conventional sites (92% and 100%); whereas, mortality was very low at one of
our organic sites (3% and 4%). Mortality among remaining sites ranged between 15% and 38%. In 2005, Spadefoot and Pacific Treefrog
(Pseudacris regilla) eggs were placed in conventional orchards (N=3) and control ponds (N=3). Our conventional sites experienced 35 - 100%
mortality; whereas our reference sites experienced less than 12% mortality.
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CAN THE TIMING AND LOCATION OF WETLAND HABITAT ENHANCEMENT INCREASE THE SUCCESS OF THE NORTHERN LEOPARD
FROG RECOVERY PROJECT ON THE CRESTON VALLEY WILDLIFE MANAGEMENT AREA, IN SOUTHEASTERN BRITISH COLUMBIA?/
EST-CE QUE LE TIMING AINSI QUE LE SITE DE PROJETS D'AMÉLIORATION DES TERRES HUMIDES SUR LE CRESTON VALLEY
WILDLIFE MANAGEMENT AREA PEUVENT AUGMENTER LE SUCCÈS DU PROJET DE RECOUVREMENT DE LA GRENOUILLE LÉOPARD
DANS LE SUD-EST DE LA COLOMBIE-BRITANNIQUE?
Marc-André Beaucher 1 and Doug Adama2
1Creston Valley Wildlife Management Area, Box 640, Creston, B.C., V0B 1G0, phone: (250) 402-6902, fax: (250) 402-6910,
[email protected]
2Adama Wildlife, Box 158, Golden, B.C., V0A 1H0, phone (250) 348-2366, [email protected]
Until the spring of 2005, northern leopard frogs (Rana pipiens) in British Columbia were confined to a single location in the Creston Valley Wildlife
Management Area (CVWMA). As part of a routine wetland management strategy to maintain waterfowl habitat in the CVWMA, approximately 45
hectares of wetland, called Leach Lake unit #4, were treated in 2004. Thick continuous stands of emergent vegetation of Typha latifolia and
Scirpus sp. were reduced by mechanical mowing, baling, and tilling, and large areas of shallow water (<1 m deep) previously encroached on by
emergent vegetation were opened up. In the spring of 2005, several male R. pipiens were heard calling in the open shallow areas created in that
unit in 2004. Subsequently, three egg masses were discovered in the same area. Although R. pipiens likely existed in this unit in the past, there
were no official records up until 2005. Two of three calling males captured during spring nocturnal surveys, turned out to be animals that had
been reared in captivity in 2003 as part of the Northern Leopard Frog Recovery Project. Marked with Visual Implant Elastomer, these two frogs
had been released more than 2 km away. These results suggest that recovery efforts may need to consist of more than a single approach. A
habitat enhancement project is in progress in unit 2b of Corn Creek marsh (summer 2005). Will frogs released in previous years move into this
area and breed?
Jusqu'au printemps de 2005, les grenouilles léopards (Rana pipiens) en Colombie-Britannique étaient restreintes dans une seule région soit celle
du Creston Valley Wildlife Management Area (CVWMA). En 2004, dans le cours du programme d'aménagement des terres humides pour
préserver l'habitat des oiseaux aquatiques sur le CVWMA, environ 45 hectares de terres humides dans l'unité Leach Lake #4 ont été traitées.
Des grandes étendues de végétation, spécialement Typha latifolia et Scirpus sp. ont été éliminées par fauchage, mise en balles et ensuite
enfouies par labourage. De grandes aires d'eaux peu profondes (<1 m) jusqu'alors envahies par la végétation émergente ont pu être rétablies.
Au printemps de 2005, on a pu entendre le chant de plusieurs mâles R. pipiens dans les eaux peu profondes rétablies dans cette unité en 2004.
Trois masses d'�ufs furent par la suite découvertes dans cette même zone. Malgré le fait que R. pipiens étaient probablement présent dans
cette unité auparavant, aucune mention officielle n'avait été enregistrée antérieurement à l'année 2005. Deux des trois mâles capturés lors d'une
visite nocturne du site le printemps dernier se sont avérés être deux individus élevés en captivité en 2003 dans le câdre du Project de
Recouvrement de la Grenouille Léopard . Portant une marque de couleur dans une des palmes arrières (Visual Implant Elastomer), ces deux
grenouilles avaient été relâchées à une distance de plus de 2 km du nouveau site. Ces résultats nous portent croire que les efforts de
recouvrement puissent nécessiter plus qu'une approche unique. Un project d'aménagement de terres humides est en cours dans l'unité Corn
Creek 2b (été 2005). Est-ce que les grenouilles rélâchées dans les années précédentes vont s'implanter dans cette unité et se reproduire?
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A COMPARISON OF PREFERRED AND OPTIMAL TEMPERATURES IN THE COMMON MAP TURTLE (Graptemys geographica) /
COMPARISON DE LA TEMPERATURE PRÉFÉRENTIELLE ET DE LA TEMPERATURE OPTIMALE CHEZ LA TORTUE GÉOGRAPHIQUE
(Graptemys geographica)
Elad Ben-Ezra1, Gregory Bulté2, Gabriel Blouin-Demers3
1Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, ON, Canada, K1N 6N5. Telephone: (613) 562-5800-2574. Fax: (613)
562-5486. E-mail address: [email protected]
2Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, ON, Canada, K1N 6N5. Telephone: (613) 562-5800-2574. Fax: (613)
562-5486. E-mail address: [email protected]
3Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, ON, Canada, K1N 6N5. Telephone: (613) 562-5800-6749. Fax: (613)
562-5486. E-mail address: [email protected]
According to the thermal coadaptation hypothesis, the preferred temperature (Tset) of ecotherms should match their optimal temperature (To) for
performance. Moreover, indices of thermoregulation assume that Tset and To are the same. We tested both this prediction and assumption with
male common map turtles (Graptemys geographica) by determining Tset and To for swimming speed and righting time. The first goal of this
study is to compare Tset measured by two methods: a dry thermal gradient and an aquatic basking arena. The second goal of this study is to
compare Tset obtained by these two methods to To for the two locomotory performances. Given that map turtles are highly aquatic, we predict
that Tset determined in the basking arena will better represent To, since moisture may be a limiting factor in the dry gradient, resulting in lower
selected temperatures. In addition to testing the thermal coadaptation hypothesis with turtles for the first time, this study also has implications for
the use of thermoregulation indices to study thermoregulation in free ranging reptiles.
Selon l'hypothèse de la coadaptation thermique, la temperature prérentielle (Tset) des ectothermes devrait correspondre à leur temperature
optimale (To). De surcroît, les indices de thermoregulation assument que Tset et To sont les mêmes. Nous avons testés cette prediction et
présomption avec des mâles tortues géographique (Graptemys geographica) en déterminant Tset et To pour la vitesse natatoire et de
redressement. Le but premier de cette étude est de comparer Tset mesuré avec deux methodes: un gradient thermique et un aréna aquatique
de lézardage. Le but second est de comparer Tset obtenu avec ces deux methodes à la To des deux mesures de performance. Étant donné que
les tortues géographiques sont particulièrement aquatique, nous présdisons que Tset determiné avec l'arena de lézardage sera plus
représentatif de To puisque la faible humidité dans le gradient themique pourrait amener les tortues à selectionner des temperatures plus
basses. En plus de tester l'hypothèse de la coadaption thermique pour la première fois chez un chélonien, cette étude comporte aussi des
implications concernant l'utilisation des indices de thermoregulation.
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WHAT IS CRITICAL HABITAT? THE CASE OF THE JEFFERSON SALAMANDER
Karine Beriault, J.P. Bogart
University of Guelph, Ontario, Department of Integrative Biology, 519-824-4120 x56260, [email protected]; [email protected]
With the recent advent of legislation such as the Species at Risk Act (SARA; 2003), scientists and Recovery Team members have been given
the thankless task of identifying and describing critical habitat for Canada's threatened and endangered wildlife. Putative habitat variables and an
information-theoretic approach (AIC) to modelling have recently become popular tools used to accomplish this. I will argue that the AIC approach,
regardless of its potential, can sometimes provide us with a limited amount of novel information. In 2004-2005, I used this method to describe
Jefferson Salamander (Ambystoma jeffersonianum) habitat throughout its range in southern Ontario but got no significant results. Using my study
and others, I will discuss the benefits and limitations of AIC as a method for identifying and describing critical habitat.
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THERMOREGULATION, HABITAT USE, AND FITNESS IN REPTILES (Plenary Address)
G. Blouin-Demers
Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa (Ontario), K1N 6N5, Canada, 613-562-5800 x6749, [email protected]
Most physiological processes depend on temperature. Because terrestrial ectotherms do not generate their own body heat, they adjust their body
temperature behaviorally (by preferential use of habitats) to maintain optimal performance. Thus, there should be a particularly tight link between
thermoregulation, habitat selection and fitness in ecotherms. The cost-benefit model of thermoregulation predicts that ectotherms should invest
more in thermoregulations when the costs are low, but contrary to this prediction, I showed that ectotherms invest more in thermoregulation when
the costs are high. I demonstrated that snakes use habitats selectively to thermoregulate: snakes that thermoregulate more use edges more, and
edges have the highest thermal quality of all available habitats. How appropriate patterns of habitat use, driven by thermoregulation, result in
increased fitness remains unclear. I used operative environmental temperatures and the body temperatures of free ranging snakes to model the
performance that would result from random habitat use; this allowed to estimate the benefit of thermoregulation.
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ÉTUDE DES OUAOUARONS DANS LES SOUS-BASSINS VERSANTS DE LA RIVIÈRE YAMASKA: MISE EN CONTEXTE ET QUALITÉ DE
L'EAU / INVESTIGATION OF BULLFROGS IN SUBWATERSHEDS OF THE YAMASKA RIVER: GENERAL INTRODUCTION AND WATER
QUALITY
Monique H. Boily1,5, Philip A. Spear1,5, Guillaume B. Cardin1,5, Daniel Rivest1, Philippe Juneau1,2,5, Christian DeBlois3,5, Nathalie Dassylva3,
Isabelle Giroux4 et Denis Laliberté4
1Département des sciences biologiques et Centre de recherche TOXEN, Université du Québec à Montréal; C.P. 8888, Succursale Centre-Ville,
Montréal, Québec, Canada, H3C 3P8, Téléphone: (514) 987-3000, poste 4346, Courriel: [email protected] Télécopieur: (514) 987-4647
2Chaire de recherche du Canada en écotoxicologie des microorganismes aquatiques
3Centre d'expertise en analyse environnementale du Québec, Ministère du Développement Durable, de l'Environnement et des Parcs du Québec
4Direction du suivi de l'environnement, Ministère du Développement Durable, de l'Environnement et des Parcs du Québec
5Réseau de recherche en écotoxicologie du Saint-Laurent
L'agriculture est susceptible d'avoir plusieurs impacts sur les amphibiens en affectant, entre autres, la qualité de l'eau. Afin de préciser les
caractéristiques des eaux de surface, des échantillons on été collectés en 2004 dans six sous-bassins de la rivière Yamaska qui traverse la
région agricole la plus importante du Québec. Les sous-bassins représentaient différentes activités agricoles - soit faible, moyenne ou intensive.
L'échantillonnage a été répété quatre fois entre juin et juillet. Chaque échantillon a été analysé pour 53 pesticides, des composés azotés et
phosphorés, la biomasse des algues, la présence de cyanobactéries ainsi que des paramètres physico-chimiques standard. La qualité de l'eau a
été comparée au statut physiologique d'une espèce indigène. Le ouaouaron, Rana catesbeiana, a été choisi principalement à cause de sa taille,
sa longévité, son affinité pour l'eau et son mode d'alimentation. Les résultats démontrent que les pesticides sont davantage présents dans les
sites à forte activité agricole (rivière Noire et rivière à la Barbue). Onze pesticides étaient détectés dans au moins 17 % des échantillons, les plus
fortes concentrations étant associées à l'atrazine, le dicamba, le métolachlore et le bentazone. La concentration en nitrite la plus élevée, 429
(g/L, a été mesurée à la rivière à la Barbue et ce paramètre dépassait régulièrement la recommandation canadienne. Une grande variation de la
biomasse algale a été observée et influencée par la concentration de nutriments et de pesticides tandis que la présence de cyanobactéries était
augmentée dans les cours d'eau ayant des concentrations élevées de pesticides.
Agriculture may impact amphibian populations in many ways including changes in water quality. To investigate in detail the characteristics of
surface waters, samples were collected in 2004 from six sub-watersheds of the Yamaska River which flows through the most important
agricultural region of Québec. The sub-watersheds represented different levels of agricultural activity - low, moderate or intensive. Sampling was
repeated four times between June and July. Each sample was analyzed for 53 pesticides, phosphate and nitrogen compounds, algal biomass,
the presence of cyanobacteria and standard physico-chemical parameters. Water quality was compared with the physiological status of an
indigenous species. The bullfrog, Rana catesbeiana, was selected mainly due to its large size, long life-span, close association to water and
feeding habits. The results demonstrate that the number and concentration of pesticides are greatest in the intensive agricultural sites (rivière
Noire and rivière à la Barbue). Eleven pesticides were detected in at least 17 % of the samples. Atrazine, dicamba, metolachlor and bentazone
were typically highest in concentration. The highest nitrite concentration, 429 (g/L, was analyzed in a sample from the rivière à la Barbue, and this
parameter often exceeded the Canadian limit. Algal biomass varied widely and was influenced by nutrient and pesticide concentrations, whereas
cyanobacteria were more abundant in waters having high pesticide concentrations.
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ÉTUDE DE OUAOUARONS DANS LES SOUS-BASSINS VERSANTS DE LA RIVIÈRE YAMASKA: ÉTAT DE SANTÉ DES OUAOUARONS /
INVESTIGATION OF BULLFROGS IN SUB-WATERSHEDS OF THE YAMUSKA RIVER: HEALTH STATUS OF BULLFROGS
Monique H. Boily1,2, Philip A. Spear1,2, Anicha Nkoua1, Sylvia Ruby2,3, Catherine Dimacacos2,3, Michel Fournier2,4, Harri Salo2,4
1Département des sciences biologiques et Centre de recherche TOXEN, Université du Québec à Montréal; C.P. 8888, Succursale Centre-Ville,
Montréal, Québec, Canada, H3C 3P8, Téléphone: (514) 987-3000, poste 5605, Courriel: [email protected], Télécopieur: (514) 987-4647
2Réseau de recherche en écotoxicologie du Saint-Laurent
3Département de biologie, Université Concordia
4Institut national de recherche scientifique - Institut Armand-Frappier
Au cours de cette étude, de nombreux paramètres ont été mesurés pour évaluer l'état de santé des ouaouarons associés aux différents sous-bassins versants de la rivière Yamaska. En comparant les ouaouarons mâles des sites à l'étude, on observe des différences significatives selon
leur appartenance à des sites de faible, moyenne ou forte activité agricole. Ainsi, les ouaouarons sont plus petits là où l'agriculture intensive
domine le bassin versant (p < 0,001). De plus et de façon significative, les adultes de ces sites ont une activité cholinergique plus élevée,
présentent une dégénérescence des testicules et voient leur contingent de rétinoïdes hépatiques altéré; ratio des esters de rétinol/rétinol (p <
0,001). Au niveau du système immunitaire, on remarque une baisse: de la phagocytose, de la prolifération des lymphocytes B et T au niveau de
la rate, de la concentration sanguine des globules blancs et des protéines plasmatiques. Pour les têtards de ouaouarons capturés dans quatre
des six sites, on observe des anomalies dans le développement des testicules pour trois de ces sites. En général, les grenouilles associées aux
sites d'agriculture intensive semblent présenter un bilan de santé déficient. D'autres paramètres font également partie de l'étude : parasitologie,
bactériologie, métaux, BPC, estimation de l'âge, bio-essai de différenciation cellulaire et hormones. L'ensemble des données sera évalué afin de
déterminer quels sont les meilleurs biomarqueurs pouvant être testés en regard de la contamination agricole pour les ouaouarons et,
possiblement, pour d'autres espèces amphibiennes.
In the course of this investigation, numerous parameters were measured in order to evaluate the health of bullfrogs in different sub-watersheds of
the Yamaska River. Comparison of male bullfrogs revealed significant differences between sub-watersheds in relation to low, moderate or
intensive agricultural practices. Thus, adult males are smaller where intensive agriculture predominated (p < 0.001). In addition, adults at these
sites have elevated cholinesterase activity, testicular degeneration and an alteration of the ratio of retinyl esters /retinol in the liver (p < 0.001).
With respect to the immune system, bullfrogs collected from the intensive agriculture sites demonstrated a diminution of phagocytosis, decreased
splenic lymphocyte B and T proliferation and lower white blood cell counts. In the case of tadpoles, testicular development was impaired at three
of four sites investigated. A general decrease in bullfrog health is associated with sites characterized by intensive agriculture. The study includes
several other parameters: parasitology, bacteriology, metals, PCBs, age determination, bio-assay of cellular differentiation and hormones.
Overall, the results will be used to identify the best biomarkers for agricultural pollution in bullfrogs and possibly for other amphibians.
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FORESTRY GUIDELINES TO CONSERVE RARE AMPHIBIANS AND REPTILES: A CASE STUDY FROM MASSACHUSETTS
Leslie Bol and Henry Woolsey
Massachusetts Natural Heritage and Endangered Species Program, MA Division of Fisheries & Wildlife, 1 Rabbit Hill Rd, Westborough, MA
01581, U.S.A, Ph: 508-792-7270 x302 Leslie Bol , Ph: 508-792-7270 x162 Henry Woolsey, Fax: 508-792-7821, Email: [email protected],
[email protected]
Close to 60 percent of Massachusetts consists of forested land, ranking 8th in the US in percentage of forest cover. Conversely the population
density is the 3rd highest and forested habitat is constantly being lost mainly to residential development. The state has 22 listed rare amphibian
and reptile species. Habitat that is important for these species is protected under the Massachusetts Endangered Species Act (MESA) and has
been identified on Priority Habitat Maps developed by the Natural Heritage and Endangered Species Program (NHESP). Applicants for any
project or activity that fall within Priority Habitat and could potentially result in a "take" must file with the NHESP. However, a properly filed,
NHESP-approved, and implemented Forest Cutting Plan is exempt from the normal filing requirements of the MESA regulations. Currently the
NHESP is developing forestry conservation management practices (CMPs) guidelines for 4 turtle species and 5 salamander species. These
guidelines are being developed in collaboration with practicing foresters and forest-managing state agencies. The objective of these
recommendations is to protect rare species populations and maintain rare species habitat for long-term viability while allowing for the sustainable
management of forests. The CMPs will help to bring together the latest science available, improve consistency of NHESP comments on Forest
Cutting Plans and will make the outcome of NHESP review of the Forest Cutting Plans more predictable to the forestry community. The process
of developing the CMPs, their specific recommendations, the challenge of balancing rare species conservation with the economic need for
private landowners to harvest timber, and the overall conservation benefit of private land being retained as forest will be discussed.
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THREATS AND LIMITING FACTORS TO NESTING AND EMBRYO HATCH SUCCESS OF THE SPINY SOFTSHELL
Ryan M. Bolton and Ronald J. Brooks
Department of Integrative Biology, University of Guelph, Guelph, ON. N1G 2W1, Telephone: 519-824-4120 X 3944, Fax: 519-824-1656, Email:
[email protected]
The Spiny Softshell (Apalone spinifera), a freshwater turtle, has a very limited range in Canada and is restricted to only a few primary populations
and nesting sites (Rondeau Provincial Park, Thames River, Long Point NWA, and Lake Champlain). A primary limiting factor for this species'
population maintenance is reproductive success. If there is no influx of hatchlings into the population then there can be no recruitment and the
population will be eliminated inevitably. Spiny Softshells are primarily aquatic and emerge to nest on a handful of small beaches at Long Point,
Rondeau, Thames River, and Lake Champlain. Research is currently being carried out at the Long Point nesting beach in order to provide a
comprehensive examination of nesting behaviour, nest site selection including microhabitat variation of nest sites, sources and rates of nest
depredation, and embryo hatch success of the Spiny Softshell. Preliminary results indicate that human recreation is a significant limiting factor of
Spiny Softshell nesting activity. Correspondingly, nest site variation (nest chamber temperature, distance to shore, surrounding vegetation
density, etc.) is a factor that appears to affect embryo hatch success. Another threat to this population is depredation of nests by a species of
flesh fly (genus Sarcophaga). The flesh fly has the potential to become a serious threat to the populations of Spiny Softshell in Ontario and the
severity of flesh fly depredation is also being studied at Long Point.
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ACCOUNTING FOR VARIABILITY IN A POPULATION VIABILITY ANALYSIS OF NOVA SCOTIA'S BLANDING'S TURTLE (Emydoidea
blandingii)
Guillaume Bourque1, T. B. Herman1, J. A. McNeil1, and D. D. Hurlburt2
1Department of Biology, Acadia University, Wolfville, Nova Scotia, B4P 2R6,
2Bowater Mersey Paper Company, Liverpool, Nova Scotia
2Mersey Tobeatic Research Institute, P.O. Box 215, Kempt, Nova Scotia, B0T 1B0
1902-585-1469 2902-682-2371 phone
1902-585-1059 2902-682-2760 fax
[email protected] , [email protected] , [email protected] , [email protected]
In Nova Scotia, Blanding's turtle (Emydoidea blandingii) exists as a spatially structured population complex (with at least three distinguishable
populations), which is designated both nationally and provincially as "Endangered" (respectively by COSEWIC and NSESA). This project
represents Phase 2 of a Population Viability Analysis (PVA) for the species in southwest Nova Scotia. The ultimate goal is to conduct a realistic
PVA for a long-lived, late maturing species that will integrate genetic, habitat and demographic parameters for the entire Nova Scotia Blanding's
turtle population complex. We limit our efforts in this study to the Kejimkujik National Park and National Historic Site (KNP) population only, as
fewer data are available for the other two populations in Nova Scotia. For the KNP population, we have developed a stage (age)-classified
transition matrix model using primarily internal demographic data collected over a 35-year period. Uncertainty in parameter estimates and
stochastic variability have been included in this model as sources of variation from the basic deterministic framework. This assessment indicates
that without intervention and with the current management regime, the population of Blanding's turtle at Kejimkujik National Park will decline.
Although the capacity to increase adult survival in this population is limited, the addition of management regimes that enhance the survival of
early life-stages (laboratory incubation and hatchling headstarting) has the capacity to enhance long-term persistence of the population.
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ESTIMATION DE L'ÂGE DES OUAOURONS PAR SQUELETTOCHRONOLOGIE/AGE ESTIMATION IN THE BULLFROG USING
SKELETONOLOGY
Marie-Lou Breton1,2, Philip A. Spear1,2, Marc Levasseur2 et Monique Boily1,2
1Département des sciences biologiques et Centre de recherche TOXEN, Université du Québec à Montréal; C.P. 8888, Succursale Centre-Ville,
Montréal, Québec, Canada, H3C 3P8, Téléphone: (514) 987-3000, poste 5605, Courriel: [email protected], Télécopieur: (514) 987-4647
2Réseau de recherche en écotoxicologie du Saint-Laurent
Dans le cadre des expériences sur l'impact de la contamination agricole sur le ouaouaron (Rana catesbeiana), des différences importantes de la
taille et du poids ont été constatés. Plus particulièrement, les mâles associés à un sous-bassin de la rivière Yamaska, caractérisé par une forte
activité agricole (rivière à la Barbue), sont significativement plus petits. Cet effet peut être dû à une croissance ralentie par la contamination du
milieu ou encore parce que les individus de ce site sont plus jeunes. L'âge des ouaouarons devient alors un facteur déterminant. L'estimation de
l'âge chez les amphibiens peut se faire en utilisant la squelettochronologie, une technique qui consiste à compter les anneaux de croissance
révélés par la coloration d'une coupe transversale d'un os, le plus souvent une phalange. L'application de la technique sur les ouaouarons de
notre étude a permis, dans un premier temps, d'établir des classes d'âge. Les premiers résultats tendent à démontrer que les ouaouarons de 6
ans et plus sont plus fréquents dans les sites ayant une faible activité agricole alors qu'une majorité d'individus plus jeunes (2 ans et moins)
occupent les sites plus contaminés. Bien qu'aucune publication ne le confirme, la squelettochronologie appliquée au ouaouaron est controversée
due au phénomène de résorption osseuse caractéristique des espèces longévives. Nous travaillons actuellement à améliorer la méthode afin
d'acquérir davantage de précision: comparaison des coupes des phalanges avec des os longs, colorants, décalcifiants, etc. L'estimation de l'âge
des ouaouarons va permettre une meilleure interprétation des paramètres morphométriques mesurés sur le terrain.
As part of experiments into the effects of agricultural contamination, large differences in body mass and length were observed in the bullfrog
(Rana catesbeiana). More specifically, males collected from a sub-watershed of the Yamaska River associated with intensive agricultural
practices (rivière à la Barbue) were significantly smaller than bullfrogs collected from other sites. This effect may be explained by several
phenomena including a decrease in growth rate or because males at this site are younger. Establishing the bullfrogs' age is therefore a logical
next step. Generally, the estimation of age for amphibians can be achieved by skeletochronology, a technique which involves counting growth
rings in stained sections of transverse cuts of bone - typically taken from the toe. Applying this technique to bullfrogs in our study allowed the
identification of age classes. Preliminary results indicate that bullfrogs of at least six years of age are found in sub-watersheds associated with a
low level of agricultural activity. Younger bullfrogs (2 years or less) populate the moderate and intensive agricultural sites. While no scientific
publication was found, skeletochronology is nonetheless thought to be unsuitable for bullfrogs due to the problem of bone resorption encountered
in long-lived species. We are presently attempting to improve the method by comparing sections of the toe to those of long bones, testing stains
and decalcification solutions. The data will contribute to the interpretation of morphometric variables of bullfrogs measured in-field.
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RATTLESNAKE CONSERVATION IN THE SOUTH OKANAGAN VALLEY
J. Brown1, C. A. Bishop2, B. Baptiste3, M. Sarell4, S. Austen5, M. Holm6
1University of Guelph, Dept. Zoology, Guelph Ontario. [email protected]
2Canadian Wildlife Service, 5421 Robertson Rd, Delta, BC V4K 3N2 [email protected] ; 604.940.4671 office phone; 604.220.2531 cell;
604.946.7022 fax
31000 Rancher Creek Rd, RR 1, Site, 53, C1, Osoyoos, BC, Canada V0H 1V0, 250-495-7901 ; 250-495-7912 fax. [email protected]
4Ophiuchus Consulting, RR #2 S53A C4, Oliver BC V0H 1T0, 250 498 6118 , [email protected]
5South Okanagan-Similkameen Stewardship Program, [email protected], (250) 492-7196
6Cannings and Holm Consultants, 1330 Debeck Road, S11, C96, RR#1, Naramata, BC V0H 1N0 250-496-4049. [email protected]
Conservation of snakes, particularly Northern Pacific rattlesnakes, is now a significant focus of research by First Nations and environmental
organizations who are conducting outreach to farmers, and park operators in the south Okanagan. Who would have thought 5 years ago,
rattlesnakes would not only be a cornerstone of a major ecotourism centre in the south Okanagan, but that vineyards would be erecting snake
friendly fencing? In 2004, the Nk'Mip Desert and Cultural Centre expanded a mark-recapture study started in 2002 on Osoyoos Indian Band
lands in the southern Okanagan valley. Since its beginning they have marked over 450 individual rattlesnakes in a natural grassland site
bordered by vineyards, golf course and campground. The rattlesnake is a major focus of tours and education programs and tourists really come
there to see rattlers. It is an ideal area to study the impacts of various threats to rattlesnakes, in particular, the impact of translocating snakes to
protect humans and yet not kill snakes. In 2005, the project identified 3 new den sites among 15 identified to date through the project, telemetry
tracked a total of 33 snakes. The Centre will host several workshops for park operators to address issues around snake handling and
translocation in parks. The Land Conservancy and Okanagan Similkameen Conservation Association has been working with vineyards to reduce
snake mortality by creating snake friendly fencing and training agricultural workers to handle and identify snakes. This year, a particular focus will
be to approach farmers for whom English is a second language by translating pamphlets on snake conservation into Punjabi and conducting on
site field workshops with them.
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IDENTIFYING HABITAT FEATURES AT LOCAL AND LANDSCAPE SCALES THAT AFFECT THE DISTRIBUTION AND ABUNDANCE OF
ANURAN AMPHIBIANS IN THE WESTERN BOREAL FOREST
Constance L. Browne, Shelly Boss, A. Lee Foote, and Cynthia A. Paszkowski
University of Alberta, Edmonton, AB, Canada, email: [email protected]
Habitat alteration is recognized as the greatest threat to amphibian populations in North America, however, the habitat requirements of many
species are poorly known. Habitat requirements must be identified in order to create management plans to preserve or restore amphibian
populations. The goals of our study were to (1) examine relationships between the relative abundance of three anuran species and habitat
features for 24 ponds in boreal Alberta, and (2) determine what spatial scale is most appropriate for predicting patterns of abundance. Our work
was part of a multi-disciplinary investigation, the HEAD (Hydrology, Ecology, And Disturbance) research project. HEAD collected detailed data on
the 24 study ponds with the goal of creating a GIS based Decision Support System to predict the responses of boreal wetlands to natural and
man-made disturbances. Ponds were chosen to represent wetlands on three major landforms: glacial lacustrine deposits, outwash plain, and
moraine. We conducted standardized visual surveys for anurans along the shoreline of each of the 24 HEAD ponds from May to August 2004.
Anuran relative abundance was estimated using catch/effort data (# hand captures/time searching) for the wood frog (Rana sylvatica), boreal
chorus frog (Pseudacris maculata), and western toad (Bufo boreas). Data on terrestrial and aquatic vegetation, invertebrate abundance, pond
physical features, beaver activity, and water chemistry were used to characterize local environments. Using GIS, the percentage of different land
cover types were estimated in areas 100 m, 500 m, and 2000 m around each pond to characterize the terrestrial landscape at three scales.
Multivariate ordination analyses were used to determine which habitat variables were most effective at explaining patterns of anuran abundance
and assemblage structure at each of the four scales. Conductivity and invertebrate biomass were significant at the local, pond-level. Proportion of
closed conifer, closed deciduous, urban/agriculture, and young, post-fire stands played a significant role in the landscape-level analyses.
Partitioning of variance indicated that local, pond variables and landscape variables were both important in explaining patterns of anuran species
abundance. Of the three landscape scales, the nature of land cover in a zone extending 500 m around breeding ponds was more effective at
explaining the structure of anuran assemblages than cover in 100 m or 2000 m zones. At a macroscale, landform also influenced the nature of
wetland habitats and amphibian assemblages. Our study suggests that management strategies incorporate local and landscape scales to
conserve aquatic and terrestrial habitats of amphibians in the western boreal forest.
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QUANTIFYING AGE AND SEX SPECIFIC ZEBRA MUSSELS PREDATION BY THE COMMON MAP TURTLE (Graptemys geographica) USING
STABLE CARBON ISOTOPES: PRELIMINARY RESULTS
Grégory Bulté and Gabriel Blouin-Demers
Dép. de Biologie / Dep. of Biology, Univ. d'Ottawa / Univ. of Ottawa, 150 Louis Pasteur, Ottawa (Ontario), K1N 6N5 Canada, T: (613) 562-5800-6749 F:(613) 562-5486, [email protected], [email protected]
In common map turtles (Graptemys geographica), females are mollusc specialists, whereas males have a broader diet. The invasion of zebra
mussels (Dreissena polymorpha) has disrupted the trophic structure and mollusc fauna of freshwaters ecosystems and may have altered the
trophic ecology of common map turtles. We used stable carbon isotopes to investigate the trophic ecology of common map turtles in a lake seve
rely infested by zebra mussels. The mean 13C of zebra mussels was -29.430. Other prey items (Gastropoda, Trichoptera, Ephemeroptera) add
a mean 13C value of 20.000. Mean 13C values of adult males, juvenile females (overlapping in size with males), and adult females were -20.33�, -22.26� and -24.86 � respectively. A two-source mixing model showed that the diet of males, juvenile females and adult females was
composed of pelagic prey at 5%, 24%, and 52% respectively. Our results show that zebra mussels are an important component of map turtles
diet but their impact on map turtles ecology is yet to be investigated.
Chez les tortues géographiques (Graptemys geographica), les femelles préfèrent les mollusques alors que les mâles ont une diètes plus
diversifiées. L'introduction de la moule zébrée a altérée la structure trophique et la faune de mollusques des lacs et rivières et pourrait avoir
affectée l'écologie trophique des tortues géographiques. Nous avons utilisés les isotopes stables du carbone pour investiguer l'écologie trophique
des tortues géographiques dans un lac infester par les moules zébrées. Le 13C moyen des moules zébrées étaient -29.430 et 20.000 pour les
autres proies (Gastropodes, Trichoptères et Éphémeres). Le 13C des mâles adultes, des femelles juveniles et des femelles adultes étaient de -
20.33�, -22.26� et -24.86 � respectivement. Un modèle de mélange démontre que la diète des mâles, des femelles juveniles et des femelles
adultes est composée de moules zébrées à 5%, 24%, et 52% respectivement. Nos résultats démontrent que les moules zébrées sont une
composante importante de la diète des tortues géographiques mais leur impact sur l'écologie des tortues demeure, cependant, à investiguer.
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EFFECTS OF COMMERCIAL FISHING TRAPS ON A MAP TURTLE (Graptemys geograhica) POPULATION IN THOMPSON'S BAY, ST-LAWRENCE RIVER
Marie-Andrée Carrière
Dép. de Biologie / Dep. of Biology, Univ. d'Ottawa / Univ. of Ottawa, 150 Louis Pasteur, Ottawa (Ontario), K1N 6N5 Canada, FAX:(613) 562-5486, Email: [email protected]
A population of map turtles (Graptemys geographica) along Grenadier Island in the St-Lawrence River is presently being studied to determine
movement patterns and habitat use. Since May 1st 2005, 175 individuals have been captured, measured, and individually marked. Of these, 12
adult females and 3 juvenile females are being tracked regularly using radio-telemetry. By mid-May the majority of the turtles had moved in
Thompson's Bay on the north side of the river where 9 commercial hoop net traps were set. We tracked turtles directly to these traps on six
occasions and have had 15 confirmed occurrences of map turtles within the traps. Because the nets were set in murky waters and we were
forbidden by law to manipulate the traps, we cannot accurately determine the actual capture rates of turtles within the traps. These commercial
hoop traps were completely submersed and this resulted in the drowning and death of the trapped turtles. A total of 15 (3 males, 12 females)
dead map turtles were found along the northern shoreline near the traps, including 3 of our turtles with transmitters. In addition, 7 dead snapping
turtles (Chelydra serpentina) and 9 dead painted turtles (Chrysemys picta) were also seen and we have rescued a trapped stinkpot (Sternotherus
odoratus) from one of the traps. The typical western winds on the river likely carried away many other dead turtles into the main river system
instead of washing them up on shore, which suggests that mortality numbers were probably much higher than what was recovered. These map
turtle mortalities represent 8.6% of our total (175) captured individuals. Map turtles are designated as Special Concern by COSEWIC and,
therefore, such high mortality rates are of serious concern to the sustainability of a population that is already in decline. This population is also
affected by the additional pressure of Highway 2 that borders the north shore of Thompson's Bay. The highway poses a constant threat during
the nesting season. We are presently in discussion with the local Ministry of Natural Resources about possible solutions to prevent further
drowning of turtles in the area. Solutions being discussed are 1) raising of the traps to create and air space for the turtles, 2) implementation of
by-catch reduction devices to the traps or 3) prohibiting further commercial fishing in the bay. Although a solution is urgently needed for the
Thompson's Bay population, we are also interested in examining long-term solutions for commercial trapping in similar areas to prevent further
mortalities in other regions.
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LINKING SCIENCE AND STEWARDSHIP THROUGH PUBLIC EDUCATION WITH THE NOVA SCOTIA BLANDING'S TURTLE (Emydoidea
blandingii)
Brennan Caverhill and Tom Herman
Department of Biology, Acadia University, Wolfville, Nova Scotia, B4P 2R6, CANADA, 902.585.1469, [email protected],
[email protected]
The Nova Scotia (NS) Blanding's turtle population complex is small, disjunct, and subject to unique threats, which contribute to its nationally
"Endangered" (COSEWIC) and provincially "Endangered" (NSESA) status designations. The NS complex provides a diverse array of
conservation opportunities and mechanisms; of its three known populations, one occurs in a national park (Kejimkujik - KNP), one in a combined
provincially and privately protected area complex (McGowan Lake - ML), and one in a working landscape dominated by small private
landholdings (Pleasant River - PR). Although habitat in KNP and ML are effectively protected from direct human disturbance, PR is not, which
makes it an important target for public education and stewardship activities. We broadly define stewardship as an ownership of responsibility on
the part of all members of the public (not just landowners), while public education includes not only formal presentations but also random field-encounters that involve an information exchange between researchers and community members. Intensive research over the past four years
(2002-2005) in Blanding's turtle habitat surrounding the rural community of Pleasant River has resulted in the convergence of science and
stewardship, which has been facilitated by effective public education and outreach tactics. Providing education FOR the people has resulted in
supportive stewardship action FROM the people, which has been invaluable to our science and management decisions in the area.
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AMPHIBIAN DISEASES IN ONTARIO: CHYTRIDIOMYCOSIS AND RANAVIRAL DISEASE
Michelle Charbonneau1, Christina Frigden1, Michael Berrill1, and Bruce D. Pauli2
1Trent University, 1600 West Bank Drive, Peterborough, Ontario K9J 7B8, Fax (705) 748-1205, Phone (705) 748-1011 x1455, E-mail:
[email protected]
2Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Raven Road, Ottawa, ON Canada K1A OH3 , Telephone:
613.998.6690, Fax: 613.998.0458 , E-mail: [email protected]
Chytridiomycosis and ranaviral disease are two so-called emerging infective diseases (EIDs) in anuran populations that may contribute to the
decline of these sensitive species. Long term monitoring of frog populations and documentation of often subtle and brief EID outbreaks is
challenging. Infection with either pathogen does not guarantee mortality and symptoms of disease can manifest with a range of severity. Genetic
analysis suggests recent human-influenced spread yet the range and occurrence of either disease in Ontario amphibian populations is unclear.
Surveying for disease prevalence and seasonal monitoring of diseased populations are important tools in assessing the impact, local and global,
of either EID on frog declines. During a survey of disease distribution in central/eastern Ontario, centered on Peterborough and the Kawartha
Lakes region, we documented the presence of ranavirus and chytrid fungus in several Wood Frog (Rana sylvatica) and Bullfrog (R. catesbeiana)
populations, respectively. Presence of the ranavirus pathogen was confirmed in 13 of the 21 populations of Wood Frog tadpoles surveyed in
2004 and in four additional samples from the 2002 and 2003 field seasons. As well, we confirmed the first lethal outbreak of the chytrid fungus
pathogen Batrachochytrium dendrobatidis in Ontario. The die-off was first noticed in 2003 in a population of metamorphosing Bullfrog tadpoles at
Kingscote Lake, Algonquin Park. The presence of the chytrid pathogen in the Bullfrog tadpole population was confirmed in 2004 with histology
and molecular analysis using polymerase chain reaction (PCR) technology. In an associated study, because amphibians are exposed to aquatic
and terrestrial doses of pesticides that are known to have an adverse effect on components of the immune system, we are testing whether
pesticide-induced immunosuppression may help explain the range in severity of disease manifestation and mortality associated with amphibian
EIDs.
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ONTARIO'S CONSERVATION RESPONSIBILITY FOR REPTILES: THE DEVELOPMENT OF A CONSERVATION RESPONSIBILITY INDEX
BASED ON PROPORTION OF RANGE AND CURRENT CONSERVATION RANKS
Susan Cowin1, Michael J. Oldham2, Joe Cebek3
1Trent University, Peterborough ON. email [email protected]
2Natural Heritage Information Centre, Peterborough, ON. email [email protected]
3Trent University, Peterborough, ON. email [email protected]
Rare species within a jurisdiction are often targeted for conservation action. However, these species may be rare due to the fact that they are at
the edge of their geographical distribution or because they have strayed across a political boundary and not due to true global rarity. Criteria
other than rarity may be needed to identify species of conservation concern and we sought to develop a Conservation Responsibility Index (CRI)
for all extant native reptiles in Ontario. We evaluated the relative abundance of each of these species in every geopolitical region (province or
state) across its entire range to help identify those jurisdictions which are critical for the species' continued existence. Global range maps were
prepared by amalgamating published range maps using the GIS software ArcMap. The Conservation Responsibility of a jurisdiction for a
particular species was based on the proportion of a species' range that falls within said jurisdiction in combination with the conservation status
rank for the species in that jurisdiction. The development of a CRI for Ontario reptiles allowed us to prioritise and identify species which are in
need of conservation action at present, as well as species for which Ontario may have a high conservation responsibility in the future. Despite the
fact that Ontario is at the northern periphery of many reptiles' ranges, the province ranks high in proportion of range as well as Conservation
Responsibility for many of its snakes and turtles. At the species level it was found that the province ranks in the top ten jurisdictions for the CRI
for 8 out of its 15 species of snakes and for 6 out of its 8 species of turtles.
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CONSEQUENCES OF EARLY UV-B ESPOSURE ON AMPHIBIAN DEVELOPMENT AND METAMORPHOSIS
Maxine Croteau, David Lean and Vance Trudeau
Centre for Advanced Research in Environmental Genomics (CAREG), Dept. of Biology, University of Ottawa. Ottawa, ON, K1N 6N5 tel: (613)
562-5800 ext. 6015, Email: [email protected], [email protected], [email protected]
Amphibian populations have been declining worldwide since the 1960s. In some regions, it is obvious that habitat destruction and road mortalities
have devastating effects. However, many populations inhabiting what appear to be pristine aquatic environments have been in decline or have
been extirpated. There is evidence to suggest that endocrine disrupting chemicals and UV-B radiation may be linked to these declines. Our work
shows that chronic exposure (12h/day for 8 months) of developing Rana pipiens tadpoles to UV-B radiation approximating ambient springtime
water levels delayed development (approx. 1 month) and blocked metamorphosis. Although other studies have shown that UV-B can affect the
rate of development and metamorphosis of amphibians, the mechanism(s) of action of this disruption remain unknown. Our group is presently
investigating the effects of the estrogenic chemical 4-tert-octylphenol (OP) and UV-B on the thyroid system of R. pipiens tadpoles, the ultimate
mediator of amphibian development and metamorphosis. We will examine the effects of treatments on somatic growth, development and
metamorphosis in relation to thyroid histology, thyroid hormone levels and expression of deiodinase and thyroid hormone receptor genes. We
speculate that a disruption in development and metamorphosis could be a contributing factor to amphibian declines in some regions. Further
research is necessary to understand the molecular mechanisms behind the disruption in development and metamorphosis caused by UV-B
radiation.
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ARE ONTARIO REPTILES ON THE ROAD TO EXTINCTION? ANTHROPOGENIC DISTURBANCE AND REPTILE DISTRIBUTIONS WITHIN
THE PROVINCE
Joe Crowley1 and Ronald J. Brooks2
1Department of Integrative Biology, University of Guelph, Guelph Ontario, N1G 2W1, phone: (519) 780-2466 e-mail: [email protected]
2Department of Integrative Biology, University of Guelph, Guelph Ontario, N1G 2W1, fax: (519) 767-1656 phone: (519) 824-4120 ext. 53944 e-mail: [email protected]
The negative effects of roads on wildlife populations have been well documented and include habitat loss and fragmentation, road mortality,
pollution and increased human disturbance. Reptiles are especially sensitive to many of these disturbances and have undergone drastic declines
in Ontario. We hypothesized that many of the extirpations of reptile populations in Ontario are due to expanding human disturbance and therefore
predicted that road density (an indicator of human disturbance) will explain the reduced and fragmented ranges of reptile species within Ontario.
The location and status of populations of several Ontario reptile species were determined from historic records, the Ontario Herpetofaunal
Summary Atlas and personal communications. Road density was calculated surrounding each population and compared between extant and
extirpated populations. The size, location, and road density of Ontario's protected areas was also examined to determine the potential they have
to act as a tool for the conservation of reptiles within the province. Road densities associated with extirpated populations were significantly higher
than road densities associated with extant populations for all species examined. This supports the hypothesis that extirpations of reptile
populations within Ontario are associated with areas of high human activity. Park area was positively correlated with latitude: smaller than
average parks are located in southern Ontario where reptiles are most severely endangered. Road density within protected areas was positively
correlated with regional road density, indicating that many of Ontario's protected areas do not eliminate the road-associated threats that
endanger Ontario's reptiles.
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EFFECTS OF ENVIRONMENTALLY RELEVENT CONCENTRATIONS OF ATRAZINE ON GONADAL DEVELOPMENT OF SNAPPING
TURTLES (Chelydra serpentina)
Shane R. de Solla1, Pamela A. Martin1, Kimberly J. Fernie1, Brad J. Park2, Gregory Mayne1
1Canadian Wildlife Service, Environment Canada, 867 Lakeshore Road, Box 5050, Burlington, ON, L7R 4A6
2Freshwater Institute, Fisheries and Oceans Canada, Winnipeg, Manitoba, R3T 2N6 Canada
The herbicide atrazine has been suspected of affecting sexual development by inducing aromatase resulting in the increased conversion of
androgens to estrogens. We used snapping turtles (Chelydra serpentina), a species whose sex is dependent on the production of estrogen
through aromatase activity in a temperature-dependent manner, to investigate if environmentally relevant exposures to atrazine affected gonadal
development. Eggs were incubated in soil to which atrazine was applied at a typical field application rate (3.1 L/ha atrazine), ten times this rate
(31 L/ha atrazine) and a control rate (no atrazine) for the duration of embryonic development. The incubation temperature (25�C) was selected to
produce only males. Although some males with testicular oocytes and females were produced in the atrazine treated groups (3.3 to 3.7%) but not
in the control group, there were no statistical differences among treatments. Furthermore, snapping turtle eggs collected from natural nests in a
corn field were incubated at the pivotal temperature (27.5�C) where both males and females would normally be produced, and there were some
males with oocytes in the testes (15.4%). The presence of low numbers of males with oocytes may be a natural phenomenon, and we have
limited evidence to suggest that the presence of normal males with oocytes may represent a feminizing effect of atrazine. Histological
examination of the thyroid gland revealed no effect on thyroid morphology.
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COMPARISON OF TURTLE SPECIES ABUNDANCE AND RICHNESS IN DAMAGED AND RECOVERED LAKES IN SUDBURY, ONTARIO
Crystal Demmer, Tonia VanKempen, and Jacqueline D. Litzgus
Department of Biology, Laurentian University, Sudbury, ON P3E 2C6, 705-675-1151 ext 2314, Fax: 705-675-4859, Emails:
[email protected] ; [email protected], [email protected]
The Sudbury Area has been subjected to intense environmental damage to both terrestrial and aquatic ecosystems as a result of atmospheric
deposition of acid and metal pollutants during a long history of mining activities. We studied turtle species richness and abundance in two types
of lakes categorized by the degree of damage, with the intent of determining a relationship between lake water chemistry and turtle population
parameters. Because turtles feed on both vegetable and animal matter, and because these food sources are likely to bio-accumulate
environmental toxins, turtles serve as valuable bio-indicators of environmental health. We tested the hypothesis that anthropogenic pollution
would have a negative impact on turtle populations. We predicted that species richness and abundance would be lower in damaged lakes
compared to recovered lakes. Over a 5 month study period, three damaged eutrophic lakes and three recovered oligotrophic lakes were sampled
using Fyke net traps, dip nets and visual surveys. We found the opposite of what we predicted. Species richness was low in both lake types and
included only two freshwater turtle species; the painted turtle (Chrysemys picta) and the common snapping turtle (Chelydra serpentina).
Surprisingly, the abundance of both species was higher in the damaged lakes than in the recovered lakes. This ongoing study will provide
baseline information for future population and turtle species assemblage research.
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CHARACTERISTICS OF TURTLE POPULATIONS IN SMALL PONDS ALONG ROADS
Jean-François Desroches and Isabelle Picard
Collège de Sherbrooke, département des Techniques d'écologie appliquée, 475 rue du Parc, Sherbrooke (Québec), J1K 4K1 Canada , [email protected]
From 20 May to 20 August 2004, we studied Turtles by mark-recapture in five ponds along roads in the Outaouais region, Québec. Species, age
(adult, juvenile), sex and health of all turtles caught were noted. A comparison with the number and characteristics of turtles dead on adjacent
roads was made in order to evaluate the impact of this mortality on the size and sex ratio of turtle populations. Only the Painted Turtle
(Chrysemys picta) was found in sufficient numbers to do the evaluation; the Snapping Turtle (Chelydra serpentina) and the Blanding's Turtle
(Emydoidea blaindingii) were present in low density. In two ponds the number or recapture rate of Painted Turtles was too low to do an accurate
population estimation (one of them seems to be a sink for the turtles' population). At one site the annual rate of mortality of turtles on roads is
more than 10 % of the population; a decline is surely occurring at that pond if no immigration is happening, and since 1997 the Blanding's Turtle
has disappeared from that pond. At two ponds the population of Painted Turtles was estimated at 80.6�21.7 and 145.6�28.2 turtles. Only one
turtle/year is found dead on the adjacent road, so the population seems not to be in decline. The sex ratio was always near 1:1 except for a pond
where males are twice as common as females, despite very low road mortality. In Outaouais, the road mortality of turtles does locally affect some
populations of Painted Turtles, but may be more important for Snapping and Blanding's Turtles that occur in smaller populations and are less
productive (later maturity, lower densities of individuals) than Painted Turtles.
Du 20 mai au 20 août 2004, nous avons réalisé une étude de marquage-recapture sur les populations de tortues de cinq étangs situés le long de
routes en Outaouais, au Québec. Pour chaque tortue capturée, l'espèce, l'âge (adulte, juvénile), le sexe et la santé ont été notés. Une
comparaison avec le nombre et les caractéristiques des tortues trouvés mortes sur les routes adjacentes a été faite pour évaluer l'impact de
cette mortalité sur la taille et le sexe ratio des populations de tortues. La Tortue peinte (Chrysemys picta) est la seule espèce capturée en
nombre suffisant pour permettre une évaluation; la Tortue serpentine (Chelydra serpentina) et la Tortue mouchetée (Emydoidea blandingii)
étaient présentes en faible densité. Dans deux étangs le nombre ou le taux de recapture des Tortues peintes s'est avéré trop bas pour permettre
une estimation de population (l'une d'elles semble être un puits pour les populations de tortues). À l'un des sites le taux de mortalité annuelle sur
la route est de plus de 10 % de la population; un déclin survient probablement à cet étang si aucune immigration ne s'effectue, et la Tortue
mouchetée y est disparue depuis 1997. À deux des étangs étudiés la population de Tortues peintes est évaluée à 80,6�21,7 et 145,6�28,2
tortues. Une seule tortue par année est retrouvée morte sur la route adjacente dans ces cas, les populations semblent donc ne pas être
menacées de déclin. Le sexe ratio est toujours près de 1 :1 sauf pour l'un des étangs où les mâles sont deux fois plus nombreux que les
femelles, malgré une très faible mortalité sur les routes. En Outaouais, la mortalité routière affecte localement certaines populations de Tortues
peintes, mais pourrait s'avérer plus importante pour les Tortues serpentines et mouchetées, lesquelles sont présentes sous forme de populations
plus petites et sont moins productives (maturité plus tardive, densité faible).
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LOOKING FOR NESTS TO FIND THE RARE FOUR-TOED SALAMANDER (Hemidactylium scutatum) TECHNICAL APPROACH AND NEST
CHARACTERIZATION
Jean-François Desroches and Daniel Pouliot
4230 #1 Louis-Lacroix, Trois-Rivières, Québec. G8Y 5V2, 819-697-2405, [email protected], [email protected]
The four-toed salamander, Hemidactylium scutatum, is a secretive species. The species is likely to be added to the threatened or vulnerable list
for Quebec. The 1994 amphibians and reptiles Quebec Atlas reported only 22 observations. The distribution of the four-toed salamander is
provincially spread but observations are localised, leaving huge gaps between each population. Since 2001, we begin to look for nesting habitat
to find the species. The approach has permitted us to find some new populations in Outaouais, Mauricie and Quebec regions. Despite the fact
that the technique is new to Quebec's herpetologists, it has been use by many American in the early 20th century. From 2002 to 2004, we
characterized 191 four-toed salamander nests, in 14 locations. Nesting habitats were always in flat field or in small valley. Vegetation may vary
from sites to sites but some species seem to be recurrent. Nests were mainly located in moss clumps, at a mean elevation of 12.1 cm from the
water. The water below the nest was always few centimetres deep. The number of eggs by nest varied greatly. Communal nesting was observed
in 5.4% or 16.7% depending of the definition of "communal nesting" that we follow. Our results are consistent with those obtained by southern
herpetologists.
La salamandre à quatre orteils, Hemidactylium scutatum, est une espèce discrète, susceptible d'être designée menacée ou vulnérable au
Québec. L'édition de 1994 de l'Atlas des amphibians et des reptiles du Québec présente seulement 22 observations. L'espèce semble être
largement distribuée mais les observations sont très localisées et il existe de grands vides entre ceux-ci. Depuis 2001, la recherche des nids de
l'espèce nous a permis de trouver de nouvelles populations dans les regions de l'Outaouais, de la Mauricie et de Québec. Bien que cette
technique soit nouvelle pour le Québec, elle a été utilisée par quelques herpetologistes américain au début du 20e siècle. Entre 2002 et 2004
nous avons caractérisé 191 nids, répartis dans 14 populations. Les habitats de nidification étaient toujours situés en terrain plat ou encore dans
de petites vallées. La végétation dominante variait d'un site à l'autre mais certaines espèces semblent récurentes. Les nids étaient la pluspart du
temps dans des buttons de sphaigne, à une hauteur de l'eau moyenne de 12,1 cm. L'eau sous le nid était toujours peu profonde. Le nombre
d'oeufs par nids variait beaucoup. La nidification communautaire a été observée dans 5,4 ou 16,7 % des cas, dépendament de la définition que
l'on donne à l'expression de "nidification communautaire". Nos resultats sont similaires à ceux obtenus dans des régions plus au sud.
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DÉGÉNÉRESCENCE DES TESTICULES CHEZ LES GRENOUILLES OUAOURONS ADULTES (R. catesbeiana) ÉCHANTILLONNÉES DANS
LE BASSIN DE LA RIVIÈRE YAMASKA / TESTICULAR DEGENERATION IN ADULT MALE BULLFROGS (R. catesbeiana) FROM SAMPLING
SITES IN THE YAMASKA RIVER BASIN
Catherine Dimacacos1,4, Sylvia Ruby1,4, Pamela Giancola1, Monique Boily2,4, Philip Spear2,4, Michel Fournier3,4
1Département de biologie, Université Concordia, 7141 Sherbrooke O., Montréal, Québec, Canada H4B 1R6, Téléphone (514) 848-2424, poste
3428, Courriel: [email protected] ,Télécopieur (514) 848-2881
2Département des sciences biologiques et Centre de recherche TOXEN, UQÀM
3Institut national de recherche scientifique - Institut Armand-Frappier
4Réseau de recherche en écotoxicologie du Saint-Laurent
L'utilisation des pesticides en milieu agricole et leurs effets potentiels sur le cycle de reproduction des grenouilles est une préoccupation
croissante. Dans la présente étude, des ouaouarons mâles adultes ont été capturés dans 6 sites du bassin versant de la rivière Yamaska,
représentant un gradient d'activité agricole. Deborah Stairs (site 1) et la Rivière Yamaska-Nord (site 3) sont des sites de faible activité agricole,
la Rivière Pot-au-Beurre (site 2) et la Rivière Yamaska (à Farnham, site 4) sont caractérisés par une activité agricole moyenne alors que la
Rivière Noire (site 5) et la Rivière à la Barbue (site 6) sont associées à une activité intensive (maïs et de soya). L'eau de ces sites a été analysée
pour les pesticides. Pour les ouaouarons, les testicules ont été disséqués et préparés pour un examen qualitatif et quantitatif des tissus: normal
(1), dégénérescence précoce (2) et dégénérescence sévère (3). L'examen de ces tissus révèle une dégénérescence sévère pour les mâles des
sites rivière Yamaska (18,8%) et Rivière à la Barbue (26,7%) lorsque comparée au site témoin. Une dégénérescence précoce a été observée
pour les sites Yamaska-Nord (50%), Yamaska (62.5%), Rivière Noire (50%) et Rivière à la Barbue (40%). Les pesticides et principalement les
herbicides étaient présents en plus grandes concentrations dans les sites où la dégénérescence testiculaire était significativement élevée. Cette
étude est la première au Québec à rapporter une dégénérescence testiculaire chez les mâles adultes de R. catesbeiana. Les résultats suggèrent
que la reproduction des ouaouarons dans ces sites risque d'être compromise.
There is growing concern regarding the potential role which herbicides may play on amphibian reproductive cycles in agricultural zones. In the
present study, R. catesbeiana male adults were collected from sites in the Yamaska River basin, Eastern Townships, Qc, Canada. Sites studied
were, Deborah Stairs (Reference Site 1), Rivière Pot-au-Beurre (Site 2), Rivière Yamaska (Site 3), Rivière Yamaska-Nord (Site 4), Rivière Noire
(Site 5) and Rivière à la Barbue (Site 6). Water sampling was conducted at all six sites close to the time of bullfrog collection and the
concentration of 54 herbicides was determined. Testes were dissected and prepared for qualitative and quantitative histological analysis.
Accordingly, testes were categorized as (1) normal, (2) testis in early testicular degeneration and (3) testis in late testicular degeneration. Results
revealed statistically significant levels of late testicular degeneration in males at Site 4 and 6 relative to the Reference Site. Values were 18.8%
and 26.7% for Site 4 and 6 respectively. In addition there was a statistically significant difference in early testicular degeneration at Sites 3, 4, 5
and 6 relative to the Reference Site. Values of 50%, 62.5%, 50% and 40% respectively were recorded. Herbicides were found at higher
concentrations at the sites where testicular degeneration was significantly high. This is the first field study in Quebec reporting amphibian
testicular degeneration among adult male R. catesbeiana. The results suggest that adult amphibians in the Yamaska River Basin may be at risk
to irreversible reproductive effects.
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ÉTUDE TOXICOLOGIQUE PORTANT SUR LA DIFFÉRENCIATION SEXUELLE ET LA REPRODUCTION CHEZ LES TÊTARDS R.
catesbeiana ÉCHANTILLONNÉS DANS LE BASSIN DE LA RIVIÈRE YAMASKA / TOXICOLOGICAL FIELD STUDIES OF SEXUAL
DIFFERENTIATION AND REPRODUCTION IN R. catesbeiana TADPOLES COLLECTED FROM SAMPLING SITES IN THE YAMASKA RIVER
BASIN
Catherine Dimacacos1,4, Sylvia Ruby1,4, Pamela Giancola1, Monique Boily2,4, Philip Spear2,4, Michel Fournier3,4
1Département de biologie, Université Concordia, 7141 Sherbrooke O., Montréal, Québec, Canada H4B 1R6, Téléphone (514) 848-2424, poste
3428, Courriel: [email protected] , Télécopieur (514) 848-2881
2Département des sciences biologiques et Centre de recherche TOXEN, UQÀM
3Institut national de recherche scientifique - Institut Armand-Frappier
4Réseau de recherche en écotoxicologie du Saint-Laurent
Des études récentes ont démontré que des pesticides, notamment des herbicides, pouvaient jouer un rôle dans le déclin des amphibiens. Cette
étude est la première au Québec portant sur la différenciation sexuelle et le développement des gonades chez les têtards de ouaouarons. Entre
20 et 24 têtards ont été échantillonnés dans chacun des quatre sites suivants: Deborah stairs et la rivièreYamaska-Nord, deux sites ayant une
faible vocation agricole, la rivière Yamaska (Farnham), associée à une activité agricole moyenne et la rivière Noire, caractérisée par une
agriculture intensive de maïs et de soya. Lors de l'échantillonnage des têtards, des échantillons d'eau ont été prélevés dans les sites pour
l'analyse des pesticides. Le complexe rein-gonade a été disséqué et préparé pour un examen histologique des tissus. Des effets sur la
différenciation sexuelle et la reproduction sont identifiés en comparant les tissus pour la présence et l'intégrité de certaines structures ainsi que la
distinction d'étapes dans la maturation des gonades. La dégénérescence testiculaire se manifeste par un déclin de la spermatogénèse lié à la
perte de kystes à l'intérieur des lobules. Un tel déclin a été observé pour les sites Yamaska (78%), Yamaska-Nord (33,3%) et Rivière Noire
(50%) lorsque comparés au site témoin. Le profil de dégénérescence observé est en concordance avec les concentrations d'atrazine mesurées
dans l'eau de ces sites.
Current studies have demonstrated that factors such as herbicides from agricultural areas may play a role in amphibian declines. In Canada,
there are no previous toxicological field studies of sexual differentiation and gonadal development in the bullfrog tadpole R. catesbeiana. In this
study, tadpoles were collected from four sites in the Yamaska River Basin of the Eastern Townships: Deborah Stairs (Reference Site 1), Rivière
Yamaska (Site 3), Rivière Yamaska-Nord (Site 4) and Rivière Noire (Site 5). Water sampling was conducted at the sites close to the time of
tadpole collection and the concentration of 54 herbicides was determined. The kidney-gonad complex was microdissected and prepared for
qualitative and quantitative histological examination. Biomarkers were developed to establish if normal testicular development was occurring.
Results suggest there is a statistically significant decline in new cysts of germ cells entering the cycle of spermatogenesis at Site 3. In addition,
Sites 3, 4 and 5 revealed a statistically significant decline in spermatogenesis relative to the Reference Site. Testicular degeneration increased
significantly at all three sites relative to the Reference Site. Values of 78%, 33.3% and 50% were recorded at Site 3, 4 and 5 respectively relative
to the Reference Site. The pattern of testicular degeneration at all four sites was similar to atrazine levels recorded in water samples for these
sites.
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EARLY EXPOSURE TO 17�-ETHINYLESTRADIOL ALTERS SEX RATIOS AND GONADAL MORPHOLOGY OF DEVELOPING LEOPARD
FROGS (Rana pipiens)
Paula Duarte1, Natacha Hogan1, Bruce Pauli2, Michael Wade3, David R. S. Lean1, Vance L. Trudeau1
1Department of Biology, University of Ottawa, Ottawa, ON
2National Wildlife Research Centre, Environment Canada, Carleton University, Ottawa, ON
3Environmental Health Sciences Bureau, Health Canada, Ottawa, ON, Canada
e-mail: [email protected], Tel: (613) 562-5800 ext.6015
Environmental estrogens have been shown to alter gonad development, causing sex reversal, feminization or the intersex condition in some
amphibian species. Our objective was to (1) examine normal sexual differentiation during tadpole development and metamorphosis in R. pipiens
and (2) determine if the contraceptive ethinylestradiol (EE2) can have long-term effects on gonad morphology and sex ratios at metamorphosis.
Control tadpoles were exposed to acetone (0.004%) vehicle throughout the experiment. Exposure to EE2 (5nM) in water began at Gosner stage
26 (hind limb bud development). After an early, short-term exposure (STE; approximately 3 weeks) until stage 30, a subset of tadpoles was
transferred to control water while a chronic exposure (CE) continued until both groups reached metamorphic climax (stage 42). Histological
analysis of the gonads at stage 42 revealed that the sex ratio in the control group was 1:1 (female:male). STE shifted the sex ratio towards
females and increased the incidence of intersex individuals; the female:male:intersex ratio was 1:0.1:0.2. For CE, the sex ratio was 1:0.6:0.7
which suggests that the timing and length of estrogen exposure can influence the resulting sex ratios. These results indicate that a short
exposure to waterborne EE2 during the critical period of gonadal development can permanently alter sex ratios and induce intersex in a native
Canadian amphibian. Supported by U-Ottawa, NSERC, Environment Canada & Health Canada.
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BEHAVIOURAL THERMOREGULATION IN WOOD TURTLES, CHASING THE SUN SLOWLY!
Yohann Dubois, Donald Thomas, Bill Shipley
Université de Sherbrooke, Département de Biologie, Université de Sherbrooke, Sherbrooke, Qc, J1K 2R1, (819) 821-8000 ext 2084,
[email protected], [email protected], [email protected]
Almost all reptiles studied yet have an optimal temperature (Top) where the ratio between energy assimilation and expenditure is optimized,
therefore maximizing the growth rate and reproduction output. Then, behavioral thermoregulation activities, such as basking and thermal habitat
selection, aim to bring the body temperature (Tb) near the Top. We sampled available temperatures for turtles (Te) in 8 different available
habitats with a total of 25 physical models that were randomly moved within each habitat. Simultaneously, we recorded turtle's Tb (40 days, 30
minutes interval) of 18 free ranging wood turtles (6 males, 6 juveniles and 6 females, by surgically introducing a temperature recording device
(iButton, thermochron) in the backwards leg cavity and we also recorded turtle's external temperature (Text) with a thermochron fixed on the
transmitter on the carapace. Habitat thermal qualities were compared using mean habitat Te in function of hours (9h to 18h) and in function of
max Te (physical model fully exposed to the sun). Our results showed that habitats became thermally heterogeneous when max Te was above
30�C and that all habitats followed the same general daily variation pattern of Te, with a maximal difference of ~ 4�C between mean Te available
in the warmer (~24�C) (excepted the sun habitat) and cooler habitat (~ 20�C) around 14h, representing a 50% increase in metabolism for turtles
being in the warmer habitat. For every available habitats, except the one fully exposed to sun, available Te Top represented only between 0
and 2% of recorded Te in each habitat, indicating that turtles had to bask to bring their Tb close to Top. Using only the data when Te max was
above 30�C, hourly Tb distributions (9h to 18h) have been individually compared to hourly null Te distribution (representing random movement
among and within available habitats) for each turtle. The results of the Kolmogorov-Smirnov test demonstrated that Tb distribution was different
from null Te distribution, which is the necessary (but not sufficient) condition for active thermoregulation. Our results also demonstrated that
thermal habitat selection (difference between Tb and Te distributions) aimed at bring Tb closer to Top and then decreased the db value (db =
|Top - Tb|) and then the observed thermal habitat selection can be viewed as behavioral thermoregulation. Finally, there was a negative
correlation between our index of sun/shade shuttling behavior ( |Text-Tb|) and db value (|Top-Tb|), showing that turtles maintained their Tb
closer to Top by using sun/shade shuttling behavior and then chased the sun slowly.
La plupart des reptiles étudiés à ce jour ont une température optimale (Top) à laquelle la proportion entre l'assimilation et la dépense d'énergie
est optimisée, maximisant ainsi la croissance et la reproduction. Ainsi, les activités liées à la thermorégulation comportementale, tel que les bains
de soleil et la sélection thermique de l'habitat, visent à rapprocher la température corporelle (Tc) de la Top. Nous avons échantillonné les
températures disponibles pour les tortues (Te) dans 8 habitats différents avec un total de 25 modèles physiques qui étaient déplacés
aléatoirement à l'intérieur de chaque habitat. Simultanément, nous avons enregistré les Tc (40 jours, à intervalle de 30 minutes) de 18 tortues en
nature (6 mâles, 6 femelles et 6 jeunes) en implantant chirurgicalement une enregistreuse de température (iButton, thermochron) dans la cavité
de la patte arrière et nous avons également enregistré la température externe des tortues (Text) à l'aide d'un thermochron fixé sur l'émetteur qui
se trouvait sur la carapace. La qualité thermique des habitats fut comparée en utilisant la Te moyenne de chaque habitat en fonction de l'heure
de la journée (9h à 18h) et en fonction de la Te maximale (modèle physique complètement exposé au soleil). Nos résultats indiquent que les
habitats devenaient thermiquement hétérogènes lorsque la Te max était au dessus de 30�C et que tous les habitats suivaient le même patron
général de variation de Te au cour de la journée, avec une différence maximale de ~ 4�C entre les températures moyennes disponibles dans
l'habitat le plus chaud (~24�C) (excepté l'habitat au soleil) et l'habitat le plus froid (~20�C) vers 14h, ce qui représente une augmentation de 50%
du métabolisme pour les tortues se trouvant dans l'habitat le plus chaud. Pour tous les habitats disponibles, excepté l'habitat au soleil, les Te
Top représentaient seulement de 0 à 2% des Te enregistrées dans chacun des habitats, ce qui signifie que les tortues devaient prendre des
bains de soleil pour élever leur Tc près de la Top. En utilisant seulement les données lorsque Te max était supérieure à 30�C, nous avons
comparé les distribution des Tc, sé parées par heure (de 9h à 18h), aux distributions nulles de Te (représentant un déplacement aléatoire à
l'intérieur et entre les habitats) individuellement pour chaque tortue. Les résultats des tests de Kolomogorov-Smirnov ont démontré que les
distributions de Tc étaient différentes des distributions nulles de Te, ce qui constitue la condition nécessaire (mais non suffisante) pour appuyer
l'hypothèse de thermorégulation active. Nos résultats ont également démontré que la sélection thermique de l'habitat (différence entre les
distributions de Tc et Te) visait à rapprocher la Tc de la Top et diminuait ainsi la valeur de dc (dc = |Top - Tc|) et donc la sélection thermique de
l'habitat observée peut être vue comme de la thermorégulation comportementale. Finalement, il y avait une corrélation négative entre notre
indice de comportement d'alternance entre soleil/ombre ( |Text-Tc|) et la valeur de dc (|Top-Tc|), montrant ainsi que les tortues maintiennent
leur Tc près de Top en utilisant un comportement d'alternance entre le soleil/ombre et donc poursuivent lentement le soleil.
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INTERACTIVE EFFECTS OF MALATHION EXPOSURE AND RANAVIRAL INFECTIONS IN WOOD FROGS (Rana sylvatica)
Amanda L. J. Duffus1, Craig Brunetti2, Bruce D. Pauli3 and Michael Berrill4.
1Watershed Ecosystems Graduate Program, Trent University, Peterborough, ON. K9J 7B8. Tel: (705) 748 - 1011 ext. 1455, Fax: (705) 748 -
1205, email: [email protected]
2Department of Biology, Trent University, Peterborough, ON. K9J 7B8. Tel: (705) 748 - 1011 ext. 5151,Fax: (705) 748 - 1205, email:
[email protected]
3Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottawa, K1A 0H3. Tel: (613) 998 - 6690, Fax: (613) 998 -
0458, email: [email protected]
4Department of Biology, Trent University, Peterborough, ON. K9J 7B8. Tel: (705) 748 - 1011 ext. 1455, Fax: (705) 748 - 1205, email:
[email protected]
The interaction of stressors is becoming an important avenue of research permitting us to begin to understand how anthropogenic factors interact
with naturally occurring stressors. Malathion is a common pesticide used to control mosquitoes and other insects in both urban and rural areas.
Malathion is also a known immunosuppressant. Ranaviruses are a group of emerging wildlife pathogens, of which Frog Virus 3 is a member, and
are credited with contributing to the global decline of amphibians. In the wild, FV3 infections, on their own, cause a high mortality rate in wood
frog tadpoles. This experiment examined the interaction between malathion exposure and induced infections of the emerging amphibian disease
Frog Virus 3 (FV3) in wood frog (Rana sylvatica) tadpoles. Animals used in this experiment were bred in the lab from parents that were collected
in terrestrial amplexus in the wild. Both of the parents tested negatively for the virus. The tadpoles were exposed to a consistent amount of the
virus, which is known to cause infection in wood frog tadpoles, and two different levels of the pesticide in a controlled laboratory setting. It was
found that the application of a combination of a high concentration of malathion and FV3 created symptomatic viral infections after only 24 hours
post exposure/inoculation and the resulting FV3 infections were confirmed by PCR analysis. When the tadpoles were exposed to both malathion
and FV3 the mortality rate increased, with the higher level of malathion exposure having the greatest mortality rates. With a lower concentration
of malathion and FV3, mortality also increased. PCR was used to confirm the presence/absence of the virus in all trials. This study indicates that
there is a synergistic or additive interaction between malathion exposure and FV3 infections. Thus, exposure to malathion increases tadpole
susceptibility to FV3 infections.
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QUICK IDENTIFICATION OF Ambystoma HYBRIDS FROM THE JEFFERSON SALAMDER COMPLEX /IDENTIFICATION RAPIDE DES
HYBRIDES Ambystoma DU COMPLEXE DE LA SALAMANDRE DE JEFFERSON
Jeanne Dumoulin1, Sarah Noël1, Martin Ouellet2, Patrick Galois2, and François-Joseph Lapointe1
[email protected], [email protected], [email protected], [email protected], [email protected]
1Département de Sciences Biologiques, Université de Montréal, C.P.6128, Succursale Centre-ville, Montréal, Québec, H3C 3J7, Canada (514)
343-6111 ext. 1031
2Amphibia-Nature, 4254 rue Garnier, Montréal, Québec, H2J 3R5, Canada, (514) 522-8105
Populations of unisexual salamanders of the genus Ambystoma can be found in eastern North America and are mainly composed of females.
These unisexual salamanders arose from ancestral hybridization events, by incorporating the nuclear genomes of four bisexual species, and
show different levels of ploidy. Although bisexual species are easily differentiated morphologically, hybrids can be difficult to discriminate visually
because they may possess intermediate characters, or morphological traits from either of their diploid counterparts. Therefore, identification
methods based on allozyme or chromosome data are often required to tell apart hybrids from bisexual species. In the present study, we introduce
a novel way to discriminate between bisexual and unisexual salamanders based on species-specific primers designed in the mitochondrial
cytochrome b region. This molecular approach provides a simple, rapid, non-invasive and efficient method, using a multiplex PCR and small
tissue samples that can easily be obtained from live specimens. This method allows for a quick identification of hybrid populations. Because of
their reproduction mode (parthenogenesis, gynogenesis or hybridogenesis), unisexual populations are more vulnerable to the loss of genetic
diversity and are therefore more susceptible to local extinction if their habitat is fragmented or altered. It is thus of primary importance to identify
hybrid populations to protect their habitat.
Les populations de salamandres unisexuées du genre Ambystoma se retrouvent dans l'Est de l'Amérique du Nord et sont principalement
composées de femelles. Ces salamandres unisexuées qui proviennent d'hybridations ancestrales, ont incorporé le génome nucléaire de quatre
espèces de salamandres bisexuées et présentent différentes combinaisons de ploïdie. Alors que les espèces bisexuées sont différenciées
morphologiquement sans ambiguïté, les salamandres hybrides peuvent posséder certains caractères intermédiaires ou traits phénotypiques
provenant de deux espèces parentales diploïdes et être difficiles à distinguer. En effet, des méthodes d'identification avec les allozymes ou les
chromosomes sont souvent requises pour différencier les hybrides des espèces parentales. Dans la présente étude, nous introduisons une
nouvelle méthode pour discriminer les salamandres unisexuées et bisexuées avec des amorces spécifiques, provenant de la région du
Cytochrome b de l'ADN mitochondriale. Cette approche moléculaire est simple, rapide, non invasive et utilise une RPC en multiplex avec des
petits échantillons de tissus pouvant provenir d'individus vivants. Cette méthode permet une identification rapide des populations hybrides. À
cause de leur différents modes de reproduction (parthénogenèse, gynogenèse ou hybridogenèse), les populations de salamandres unisexuées
sont plus vulnérables à la perte de diversité génétique et donc plus susceptibles à l'extinction locale si leur habitat est fragmenté ou altéré. Il est
donc primordial de pouvoir identifier les populations hybrides afin de bien protéger leur habitat.
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IMPACTS OF POND CONNECTIVITY ON AMPHIBIAN LIFE STAGES AT DELTA MARSH, MANITOBA
Katarzyna A. Dyszy1, Dr. Dale Wrubleski2, Dr. John Spence3
1Dept of Renewable Resources, University of Alberta, Edmonton, Alberta, Email: [email protected] , Mailing address: 638 Warsaw Avenue,
Winnipeg Manitoba, R3M 1C6, Tel: (w) 204-474-8469 (h) 204-474-2749
2Institute for Wetland and Waterfowl Research, Ducks Unlimited Canada, Stonewall, Manitoba, Email: [email protected], Mailing address:
P.O Box 1160, Stonewall, Manitoba, R0C 2Z0
3Dept of Renewable Resources, University of Alberta, Edmonton, Alberta, Email: [email protected], Mailing address: 751 General
Services Bldg, Universiy of Alberta, Edmonton, Alberta, T6G 2H5
Delta Marsh, located on the south shore of Lake Manitoba, is home to several anuran species that use the area extensively as breeding and
summering grounds. Over the past 40 years, the introduction of common carp (Cyprinus carpio) and water level stabilization have contributed to
the deterioration of those ponds connected to the main marsh while ponds isolated from the main marsh have not undergone such changes. To
study the impact of pond connectivity on anuran populations, three anuran life stages were examined at Delta Marsh. Pond connectivity may play
a key role in both breeding site selection and survival of anuran eggs. This data is supported with observations of reduced abundances of anuran
breeding choruses and egg masses in connected sites when compared with isolated sites. Connected sites also had greater physical disturbance
of artificial egg masses, suggesting where egg masses are oviposited at these sites, compounded effects from physical damage, such as from
spawning common carp found exclusively at connected sites, further reduces egg mass numbers. Tadpole abundance, growth and rate of
development was also examined with respect to pond connectivity, and found lower abundances of tadpoles in connected sites compared with
isolated sites.
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THE EFFECT OF THE CONFIGURATION OF HABITAT RELATIVE TO ROADS ON POND-DWELLING AMPHIBIANS
Felix Eigenbrod1, S.J. Hecnar2, and L. Fahrig1
1Geomatics and Landscape Ecology Research Laboratory (GLEL), Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa,
Ontario, Canada K1S 5B6 Tel: 613-520-2600 ext 3831 Fax: (613) 520-3539
2Department of Biology, Lakehead University, 955 Oliver Rd, Thunder Bay, Ontario, Canada P7B 5E1 Tel: (807) 343-8250 Fax (807) 346-7796
Email addresses of authors: [email protected], [email protected], [email protected]
Two landscape variables have been found to be particularly important in predicting the species richness of pond-dwelling amphibians - the
amount of natural habitat (forest and wetlands) and the density of paved roads present in the landscape. Many amphibians require secondary
upland habitat (usually forest), and all benefit from additional breeding habitat in the landscape (wetlands and water bodies) to allow re-colonization of breeding ponds, whereas the main effect of roads is thought to be direct mortality. However, it seems likely that a road that needs
to be crossed to access secondary habitat or other wetlands has a much greater negative effect than one that does not. In other words, the
configuration of natural habitat relative to roads is likely very important. This is not directly measured by either habitat amount or road density.
We hypothesized that the amount of natural habitat that is accessible from a breeding pond without crossing a paved road (accessible habitat
amount) will be a better predictor of amphibian species richness than the combined effects of habitat amount and road density. We tested this
hypothesis by looking at 30 pond dataset of species richness data. We found some evidence that accessible habitat was a better predictor of
amphibian species richness than the combined effect of total habitat and road density at the 500m and 600m scale. However, above the 600m
scale, total habitat and road density were highly correlated, making it impossible to tell from this dataset whether the results we found at 500m
and 600m hold at higher spatial scales. While further studies are clearly needed, these results suggest that accessible habitat amount may be
the best way to look at the combined effects of roads and habitat loss.
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DEMOGRAPHY AND BEHAVIOUR OF A GEORGIAN BAY POPULATION OF THE SPOTTED TURTLE (Clemmys guttata)
Jean Enneson, Jacqueline D. Litzgus
Department of Biology, Laurentian University, Sudbury, ON P3E 2C6, Telephone: (705) 675-1151 ext. 2314, Fax: 705-675-4859, Email:
[email protected], [email protected]
The spotted turtle is listed as Vulnerable by the IUCN, Endangered in Canada, and Vulnerable in Ontario. An island in eastern Georgian Bay is
the site of the longest-running mark-recapture study to date of the spotted turtle. Data collection has been carried out since 1977, for a total of 27
years of study. The purpose of the current study is to continue the long-term mark-recapture study to allow examination of life history,
demographic, and population size changes. From May to August of 2005, known breeding aggregations were surveyed for spotted turtles. All
turtles were marked if new, measured, weighed, sexed, injuries noted, and released. Palpation was used to determine if females were gravid and
X-rays were used to determine clutch size. Ten turtles were outfitted with radio transmitters, and their movements monitored using GPS. A total
of 33 turtles were captured in 2005; 42.4% were females, 39.4% were males, and 18.2% were juveniles. 66.7% of female turtles captured
between breeding season and nesting season were gravid (N=9). All X-rayed gravid turtles (N=3) had clutch sizes of 6 eggs. Two radio-tracked
turtles nested on a neighbouring island, which was not previously known to be a spotted turtle nesting site. The data collected from this ongoing
study will allow determination of individual survivorship, population size, and aspects of population demography, which will be applied in
population viability analyses. Ongoing radio-tracking will provide data to allow accurate calculation of home-range size. These data will be also
used to examine critical habitat shifts and breeding behaviour responses by spotted turtles to natural habitat modification (caused by beaver
damming) of a primary breeding aggregation site on the island.
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LANDSCAPE AND LOCAL FACTORS ASSOCIATED WITH VERTEBRATE ROADKILL IN SOUTHERN ONTARIO
Bob Farmer and Ron J. Brooks
Department of Integrative Biology, University of Guelph, 519-824-4120 ex. 58360, Fax 519-767-1656, [email protected],
[email protected]
From mid-May until mid-October, I am surveying roads for vertebrate roadkill in the greater-park areas of Rondeau Provincial Park and Point
Pelee National Park . By considering a variety of broad-scale and local variables, including landscape type, road density, road type, embankment
slope, shoulder type, shoulder width, road curvature, road temperature, traffic volume, weather, time of day and season, this research will identify
important determinants of roadkill for both individual and groups of species. Results will guide mitigation strategies and future construction by
identifying road designs, locations and times associated with the highest collision risk.
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ASSESSING DNA DAMAGE IN FROGS FROM AGRICULTURAL AREAS OF SOUTHWESTERN ONTARIO, CANADA
Robyn M. Ferguson1, Bruce D. Pauli1, Pamela A. Martin2, Tana V. McDaniel2 , Chris Marvin3, Loren D. Knopper4
1National Wildlife Research Centre, Canadian Wildlife Service, Environment Canada, Ottawa, ON, K1A 0H3
2Canadian Wildlife Service, Environment Canada, Burlington, ON, L7R 4A6
3National Water Research Institute, Environment Canada, Burlington, ON, L7R 4A6
4Jacques Whitford, Ottawa, ON, K1B 1A7
Genotoxicity in wildlife species as a result of non-target pesticide exposure has been reported in numerous studies. We examined whether field
exposure to agricultural chemicals might result in measurable DNA damage in ranid amphibians inhabiting areas of intensive corn-soybean
agriculture. To accomplish this we measured DNA damage in Rana pipiens (Northern leopard frogs) and Rana clamitans (Green frogs) collected
in agricultural ponds and drainage ditches in southwestern Ontario. Blood from 72 adult frogs was collected between July and October, 2004,
from 11 different sites: two control sites (without agricultural influence), one "agricultural control" site (marsh located within an agricultural area),
and eight agricultural sites. Whole blood was diluted in a cryopreservant and flash frozen in vapor phase nitrogen and subsequently stored at -80(C. At a later date, blood was thawed and DNA damage (single and double DNA strand breaks) was assessed using the alkaline Comet assay.
Water collected at the same sites as the frogs had measurable levels of atrazine, metolachlor, nutrients, and heavy metals in the agricultural and
agricultural control sites, whereas these contaminants were very low or not detected in control sites. Because of this shared contamination,
agricultural and agricultural control data were pooled and compared to control data. Measures of DNA damage using the Comet assay (tail
length, % DNA in the tail) in frogs from agricultural sites was significantly greater than in frogs from control sites (p = 0.046, 0.08). The specific
cause of the observed genotoxic damage is not known but our results suggest that frogs living in agricultural areas are exposed to genotoxins.
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CHRONIC EFFECTS OF ATRAZINE HERBICIDE ON THE DEVELOPMENT OF NORTHERN LEOPARD FROG (Rana pipiens) TADPOLES
Christina M. Fridgen1, Bruce D. Pauli2, Michael Berrill1, Kenneth Doe3 and Paula Jackman3
1Trent University Biology, Symons Campus 1600 West Bank Road, Peterborough Ontario Canada K9J 7B8, [email protected]
2Canadian Wildlife Service Ottawa Ontario
3Environment Canada Moncton New Brunswick
Amphibians are excellent model organisms in which to observe chronic effects of contaminants. They are particularly useful to examine effects of
contaminants on the thyroid and gonadal axes, because of their endocrine-mediated differentiation processes such as metamorphosis and
gonadal development. Exposure of amphibians to the popular corn herbicide atrazine has been associated with abnormal development of
exposed frogs, particularly demasculinization and feminization, through a proposed mechanism of induced aromatase activity levels increasing
conversion of androgens to estrogens. We conducted a blinded study examining the effects of chronic atrazine exposure (0.1 and 1.8 �g/L as
Aatrex Liquid 480) on the development of Northern Leopard Frog Rana pipiens tadpoles. Animals were exposed from the egg stage to
metamorphosis. Our main objectives were to compare our results with existing data, provide information concerning sensitive stages and
endpoints in Ranid amphibians, and investigate new histological methods to quantify and stage early gonad differentiation in R. pipiens. Atrazine
exposure caused significant chronic effects on both the growth and development of tadpoles and their gonads at concentrations as low as 0.1
�g/L. Preliminary statistical analyses indicate that Gosner Stage 25 to 40 tadpoles exposed to the lowest concentration tested have significantly
increased body length and mass, as well as an increased average stage of development over time, yet significantly fewer tadpoles being reared
in both test concentrations reached metamorphic climax at test termination. Both atrazine concentrations induced significantly larger female
gonads, increasing both the ovary length and area. In addition, preliminary histology has revealed signs of intersex, testes with large primary
oocytes, and ovaries with areas of abnormal somatic tissue. Further in-depth histological analysis of gonads to examine the influence of atrazine
exposure on oocyte size, gonadal differentiation rate and stages, as well as intersex incidence is being conducted.
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THE RELATIVE EFFECTS OF FORESTED, AGRICULTURAL AND URBAN LANDSCAPES ON AMPHIBIAN COMMUNITIES IN EASTERN
ONTARIO
Sara A. Gagné and L. Fahrig
Geomatics and Landscape Ecology Research Laboratory, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6
Since the early 1990s, evidence has been accumulating of the impact of landscape composition on amphibian species richness and abundance.
The present study seeks to determine the relative effects of forested, agricultural and urban landscapes on anuran species richness and
abundance in eastern Ontario. Six evening call surveys were conducted between April and June 2004 to assess anuran species richness and
abundance at ponds surrounded by > 50% forest cover (9 ponds), > 70% open cover (10 ponds), and > 50% urban cover (11 ponds) within 1.5
km radius landscapes. Vegetation and water quality surveys were also conducted at each pond in June 2004 to measure local variables likely to
affect anuran diversity. Preliminary results indicate that anuran species richness is significantly higher in forested and agricultural ponds than in
urban ponds. Pond perimeter has also been identified as a significant positive predictor of anuran species richness. The effects of forested,
agricultural and urban landscapes on the abundance of individual amphibian species will also be discussed.
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ASSESSING PREVALENCE OF CHYTRID FUNGUS (Batrachochytrium dendrobatidis) IN NATIVE AMPHIBIANS AND BULLFROGS (Rana
catesbeiana) ON VANCOUVER ISLAND, BRITISH COLUMBIA /L'ÉVALUATION DE LA PRÉDOMINANCE DU MYCÈTE DE CHYTRID
(Batrachochytrium dendrobatidis) DANS LES AMPHIBIES INDIGÈNE ET LES OUAOUARONS (Rana catesbeiana) SUR L'ÎLE DE
VANCOUVER, COLOMBIE BRITANNIQUE
Purnima P. Govindarajulu1, Trenton W. J. Garner2 and Bradley R. Anholt1
1Department of Biology, University of Victoria PO Box 3020 STN CSC Victoria, BC V8W 3N5, Canada Tel: (250) 383 6262 Fax: (250) 721 7120
Email: [email protected]
2Institute of Zoology, Zoological Society of London Regent's Park, London NW1 4RY U.K. Tel: 011 44 (0)20 7449 6687 Email:
[email protected]
Bradley R. Anholt Department of Biology, University of Victoria PO Box 3020 STN CSC Victoria, BC V8W 3N5, Canada Tel: (250) 721 7106 Fax:
(250) 721 7120 Email: [email protected]
Emerging wildlife diseases are increasingly recognized as a great threat to global biodiversity. Recently, the amphibian chytrid fungus
(Batrachochytrium dendrobatidis) has been implicated in catastrophic declines of amphibians in Europe, North America, Central and South
America, and Australia. In British Columbia, the distribution and level of prevalence of the chytrid fungus is poorly known. Important vectors of
introduction and dispersal are thought to be to be commercially traded amphibians such as the African clawed frog (Xenopus laevis) and the
American bullfrog (Rana catesbeiana). American bullfrogs have been widely introduced in the southwestern part of British Columbia and are
expanding their range. In the summer of 2005, we sampled native amphibians and bullfrogs in four bullfrog and four bullfrog-free sites on
southeastern Vancouver Island. Sensitive molecular techniques will be used to detect presence of the fungus from tissue samples of both the
aquatic and terestrial life-stages. Up to date results from this ongoing study will be presented. This study will enable us to quantify the level of
prevalence of chytrid fungus in free-ranging bullfrog populations. The level of prevalence of chytrid fungus in the non-bullfrog sites will be used to
assess background levels of infection in native amphibians. Comparison of levels of prevalence of chytrid fungus in bullfrog vs. bullfrog-free sites
will enable us to assess whether introduced bullfrogs were acting as vectors of spread of the disease.
Les nouvelles maladies de faune sont de plus en plus identifiées comme une des grandes menaces pour la biodiversité globale. Récemment, le
mycète amphibie de chytrid (Batrachochytrium dendrobatidis) a été impliqué dans des déclins catastrophiques des amphibies en Europe,
l'Amérique du nord, L'Amérique Centrale et Sud, et l'Australie. En Colombie Britannique, la distribution et le niveau de la prédominance du
mycète de chytrid est peu connue. Des vecteurs importants de l'introduction et de la dispersion sont considerés d'être les amphibiens
commercés tels que Xenopus laevis et les ouaouarons (Rana catesbeiana). Les ouaouarons ont été introduits en grand nombre au sud-ouest de
Colombie Britannique et ils sont en train d'augmenter leur domaine. En été de 2005, nous avons prélevé les amphibiens indigènes et les
ouaouarons dans quatre emplacements avec les ouaouarons, et les amphibiens indigènes dans quatre emplacement sans ouaouarons dans le
sud-est de l'île de Vancouver. Des techniques moléculaires précises seront employées pour détecter la présence du mycète dans les
échantillons de tissu tirés des specimens d'étapes aquatiques et terrestres. Les résultats le plus courant de cette étude continuelle seront
presentés. Cette étude nous permettra de mesurer la fréquence du mycète de chytrid dans les populations des ouaouarons libres en Colombie
Britannique. La frequence du mycète de chytrid dans les emplacements sans ouaouarons sera employé pour évaluer le niveau de reference de
l'infection dans des amphibiens indigènes. La comparaison de la frequence du mycète de chytrid dans les emplacements avec ouaouarons par
rapport aux emplacements sans ouaouarons nous permettra d'évaluer si les ouaouarons sont les vecteurs de la diffusion du mycète de chytrid.
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EFFECTS OF SEXUAL SIZE DIMORPHISM ON DIET SPECIALIZATION IN THE COMMON MAP TURTLE (Graptemys geographica)
Marie-Ange Gravel1, Gregory Bulté2, Gabriel Blouin-Demers3
Département de biologie - Department of Biology, Université d'Ottawa - University of Ottawa, 150 Louis Pasteur, Ottawa (Ontario) K1N 6N5
Canada, T: (613) 562-5800, F: (613) 562-5486, 1 [email protected] , 2 [email protected] , 3 [email protected]
Sexual size dimorphism may arise from sexual selection where one sex gains a reproductive advantage from being larger, or natural selection
such as diet divergence, or both. In the Common Map Turtle (Graptemys geographica), females are much larger than males, often exceeding
twice their size. Extreme female-biased sexual size dimorphism had been hypothesized to be a result of natural selection for diet divergence. The
goal of our study is to test the hypothesis that male and female map turtles specialize on different prey. We assessed prey availability in our
study lake (Lake Opinicon, Queen's University Biological Station). We collected fecal samples of adult and juvenile turtles of both sexes. Prey
size can be reconstructed from mussel septa length and snail opercula length that are passed with the feces. The two sexes appear to be
choosing different prey, as well as different prey size. Large females prey mostly on zebra mussels and snails (Viviparous georgianus), while
juvenile females and males seem to consume smaller snails, small zebra mussels and caddisfly larva (Tricoptera). Also prey size seems to
increase with body size within each sex.
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POPULATION DYNAMICS OF THE WOOD TURTLE IN THE GREATER KOUCHIBOUGUAC ECOSYSTEM, NEW BRUNSWICK /
DYNAMIQUE DES POPULATIONS DE TORTUES DES BOIS DANS LE GRAND ÉCOSYSTÈME DE KOUCHIBOUGUAC, NOUVEAU-BRUNSWICK
Mireille Gravel1, Éric Tremblay2, Tom Herman3, and Donald McAlpine4
1Kouchibouguac National Park of Canada, New Brunswick, E4X 2P1, Canada / Phone number: (506) 876-2443 / Fax number: (506) 876-4802 /
Email address: HYPERLINK "mailto:[email protected]" [email protected]
2Kouchibouguac National Park of Canada, New Brunswick, E4X 2P1, Canada / Phone number: (506) 876-2443 / Fax number: (506) 876-4802 /
Email address: HYPERLINK "mailto:é[email protected]" é[email protected]
3Biology department, Acadia University, Nova Scotia B4P 2R6, Canada / Phone number: (902) 585- 1469 / Fax number: (902) 585-1059 / Email
address: HYPERLINK "mailto:[email protected]" [email protected]
4New Brunswick Museum, New Brunswick, E2K 1E5, Canada / Phone number: (506) 643-2345 / Fax number: (506) 643-2360 / Email address:
HYPERLINK "mailto:[email protected]" [email protected]
The wood turtle and its habitat suffer important threats, linked predominantly to human activity like major deforestation, habitat fragmentation,
growing tourism and road kills, all clearly present in New Brunswick. Nevertheless, only minimal protection has been accorded in Maritime
Provinces. This turtle is widely spread in New Brunswick but of unknown abundance since no formal surveys have been undertaken. Aware of
the presence of this designated "species of special concern" reptile in Kouchibouguac National Park and its Greater Ecosystem, it becomes
crucial to act quickly by assessing the population. The specific objectives of this project are to 1) create a preliminary map of susceptible wood
turtle habitat, 2) estimate the population density, 3) investigate movements, home range and habitat selectivity and 4) elucidate reproduction
events in time and space (e.g. nesting, hatching). This project began in June 2005 and therefore, results remain preliminary. Most riparian
habitats in the region, potentially suitable for wood turtles, have been monitored. Although we cannot yet estimate population densities, we are
currently following 5 individuals by telemetry throughout the region, most likely belonging to 4 different populations. The location of hibernating
sites of these turtles will allow us to find more individuals next year and to start earlier to record reproduction events as well. By informing and
implying local communities to the project, we passed from 9 wood turtle sightings to 38 for the area and we plan on maintaining this collaboration
through the years.
Des perturbations majeures affectant la tortue des bois et son habitat telles que la déforestation, la fragmentation de l'habitat, le tourisme
grandissant et la mortalité sur la route, sont toutes clairement présentes au Nouveau-Brunswick. Toutefois, les mesures de protection accordées
à l'espèce dans les provinces maritimes demeurent minimales. Bien que cette tortue soit largement répandue au Nouveau-Brunswick, son
abondance n'est pas connue puisque aucun suivi n'a été fait dans la région jusqu'à ce jour. En sachant que ce reptile, désigné "espèce
préoccupante", est présent dans le Grand Écosystème de Kouchibouguac, il est impératif d'agir rapidement en inventoriant les populations. Les
objectifs spécifiques de ce projet sont de 1) créer une carte préliminaire d'habitats potentiels, 2) estimer la densité des populations, 3) suivre les
mouvements afin de déterminer le domaine vital et la sélectivité d'habitats et 4) identifier les événements reliés à la reproduction temporellement
et spatialement (ex. nidification, éclosion). Comme le projet a débuté en juin 2005, les résultats demeurent préliminaires. Nous avons visité la
majorité des habitats rivulaires de la région afin de déterminer la présence d'habitats potentiels pour l'espèce. Quoiqu'il soit encore impossible
d'estimer la densité des populations, nous suivons 5 individus par télémétrie dans l'aire d'étude, appartenant possiblement à 4 populations
distinctes. La localisation des sites d'hibernations de ces tortues nous permettra de trouver plus d'individus l'an prochain ainsi qu'a suivre dès
avril 2006 les événements reliés à la reproduction. En informant et en impliquant les communautés locales, nous sommes passé de 9
observations de tortues des bois à 38 dans la région et nous prévoyons entretenir cette précieuse collaboration tout au long de ce projet.
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PAVED ROADS AS BARRIERS TO AMPHIBIAN MOVEMENTS / LES ROUTES ASPHALTÉES: UNE BARRIÈRE AUX MOUVEMENTS
D'AMPHIBIENS?
Mireille Gravel1, Marc J. Mazerolle2 and Marc-André Villard3
1Canada Research Chair in Landscape Conservation, Département de biologie, Université de Moncton, Moncton, New Brunswick E1A 3E9,
Canada. / Phone number : (506) 858-4869 / Fax number : (506) 858-4541 / Email address : [email protected]
2USGS Patuxent Wildlife Research Center, Laurel, MD 20708-4017 USA / Phone number: (301) 497-5636 / Fax number: (301) 497-5545 / Email
address: [email protected]
3Canada Research Chair in Landscape Conservation, Département de biologie, Université de Moncton, Moncton, New Brunswick E1A 3E9,
Canada. / Phone number: (506) 858-4334 / Fax number: (506) 858-4541 / Email address : [email protected]
We investigated whether paved roads acted as barriers to amphibian movements in the vicinity of 16 roadside ponds of southeastern New
Brunswick, Canada. Our study was conducted over two successive summers (April-September), and focussed on three species groups: mole
salamanders, toads, and ranid frogs. Using pitfall traps and drift fences placed on each side of the pond (i.e., one fence was separated from the
pond by the road, the other was in continuous habitat, we examined the effects of roads, on 1) roadside adult and juvenile amphibian abundance
and 2) movements across the road. We measured a number of road-associated variables such as traffic intensity, road proximity to the pond,
and terrestrial habitat structure as well as others potentially influencing movements (e.g., pond size, hydroperiod). Despite considerable variation
among groups, the abundance of juveniles and adults of each group was generally negatively influenced by road-associated variables for at least
one of the study years. The proportion of juveniles dispersing from the pond was lowest at the roadside fence. In contrast, adult movements from
the pond were highest at the roadside fence under high traffic intensities. Our results strongly suggest a barrier effect of paved roads on adult
and juvenile amphibians even under low traffic densities and relatively low roadside habitat disturbances. This provides empirical evidence for
deleterious effects of roads on amphibian populations and highlights the importance of accounting for roads in amphibian management.
Nous avons étudié l'effet des routes asphaltées comme potentielles barrières aux mouvements d'amphibiens en bordure de 16 étangs le long de
routes dans le sud-est du Nouveau-Brunswick, Canada. Notre étude s'est déroulée pendant deux étés consécutifs (avril-septembre) et a ciblé
trois groupes d'espèces: les salamandres, les crapauds et les grenouilles. En utilisant des pièges-fosses et des clôtures de dérive disposées de
part et d'autre de l'étang (i.e., une clôture séparée de l'étang par une route, l'autre dans de l'habitat continu), nous avons examiné les effets de la
route sur 1) l'abondance d'amphibiens adultes et juvéniles en bordure de route ainsi que 2) les déplacements impliquant une traversée de la
route. Nous avons mesuré différentes variables associées à la route telles que l'intensité du trafic, la distance de la route à l'étang, la structure
de l'habitat terrestre ainsi que d'autres facteurs pouvant influencer les mouvements (e.g., taille de l'étang, hydropériode). Malgré les variations
considérables entre les groupes, l'abondance de juvéniles et d'adultes de différents groupes d'espèces était généralement influencée
négativement par les variables associées à la route, et ce, pour au moins une des deux années de l'étude. La proportion de juvéniles en
dispersion était plus faible à la clôture en bordure de la route qu'à celle située du côté de l'étang. Les mouvements des adultes étaient, au
contraire, plus fréquents à la clôture en bordure de la route sur les sites ayant une grande intensité de trafic. Nos résultats suggèrent fortement
un effet de barrière des routes asphaltées sur les mouvements d'amphibiens, même à faible intensité de trafic et faibles perturbations de l'habitat
en bordure des routes. Cette étude fournit donc une preuve empirique des effets néfastes des routes sur les populations d'amphibiens et
souligne l'importance de considérer les routes dans de futurs plans de gestion de ce taxon.
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THERMAL ECOLOGY OF OVERWINTERING WOOD TURTLES (Glyptemys insculpta) AT THE SPECIES' NORTHERN RANGE LIMIT
William F. Greaves and Jacqueline D. Litzgus
Laurentian University, Department of Biology, Sudbury, ON, P3E 2C6, Canada, Phone 705-670-1063, Fax: 705-675-4859,
[email protected], [email protected]
Seasonal variation in activity patterns of reptiles is accompanied by physiological and behavioural adjustments that influence both their ecology
and life history. Over-winter survival is an important factor limiting a species' northern range extension. A northern population of wood turtles
(Glyptemys insculpta) in the Sudbury District, Ontario was surveyed in fall 2004 and a subset of adults (N = 5) radio-tracked during the winter of
2004-2005 to examine thermal aspects of hibernation. We tested the hypothesis that hibernation sites that provide protection from freezing and
predation are a limited resource in the north, thus requiring turtles to use communal hibernacula. Temperature data loggers indicated that turtle
body temperatures and hibernacula temperatures remained relatively stable at -0.5�C from 2 December 2004 until 3 April 2005. During the same
period, air temperature was highly variable, ranging between -40�C and 11�C (average air temperature = -9.9�C). Turtles did not use distinct
structures (e.g., root hollows, logjams, and holes in the riverbank) that would protect from predation during hibernation, but instead rested
relatively exposed on the riverbed at a water depth of approximately 1m and at a mean distance of 0.99 m from the riverbank. Surprisingly, turtles
made small movements during winter, typically against the river current and in a direction parallel to the riverbank. Average winter home range
size was 4.40 m2. These findings contribute to a better understanding of the over-wintering ecology and thermal tolerances of G. insculpta at its
northern limit, and are especially important because G. insculpta is declining in numbers, and is particularly vulnerable to increases in adult
mortality (that may occur during hibernation) as a result of its bet-hedging life history.
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THE FOWLER'S TOAD RECOVERY PLAN
David M. Green1 and Anne Yagi2
1Redpath Museum, McGill University, 859 Sherbrooke St. W., Montreal, Quebec H3A 2K6. tel: (514) 398-4086 ext 4088. fax: (514) 398-3185.
email: [email protected]
2Ministry of Natural Resources-Niagara Area Office, 4890 Victoria Ave. North, Box 5000, Vineland Station, Ontario, L0R 2E0. tel: 905-562-1196.
fax: 905-562-1154. email: [email protected]
Although widespread throughout the eastern United States, Fowler's toad (Bufo fowleri) is restricted in Canada to sandy or rocky points and
beaches along the northern shore of Lake Erie in Ontario. Viable populations are known from only three localities immediately adjacent to the
lake: Rondeau, Long Point, and the Niagara peninsula. Assessed as "Threatened" by COSEWIC in 2000, Fowler's Toad has been legally listed
under the Species at Risk Act as of 2003. Primary identified threats to the well-being of Fowler's toads in Canada include shoreline development
and stabilization. Limiting factors include the animals' high mortality and short life span as well as the instability of its preferred shoreline habitats.
Population size fluctuations and variable connectivity between required habitats also affect population survival. With these and other
considerations in mind, the Fowler's Toad Recovery Team has crafted a recovery plan for the species based on assessment of population
viability. In rank order, recruitment of juveniles into the adult population, population number, dispersal survival and, finally, adult survival are
identified as the major factors affecting viability of Fowler's toad populations and, consequently, are the major components addressed in the
recovery plan. The heart of the plan is contained under the headings "Not Worse (Conservation)", "Know More (Research)" and "Make Better
(Amelioration and Recovery)". Outreaches to stakeholders, education, funding and reporting are also addressed as these are necessary for
ensuring the plan's successful implementation. Action items include measures to protect of existing habitat, curb the number of vehicles on the
beach, curtail aggressive beach maintenance, define critical habitat, pursue research to gain better knowledge of juvenile survivorship and
dispersal, and increase public awareness through activities, publications, toad posters, toad stickers and more.
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DEMOGRAPHIC TRAITS OF INTRODUCED COMMON WALL LIZARDS (Podarcis muralis) ON VANCOUVER ISLAND
Patrick T. Gregory
Department of Biology, University of Victoria, PO Box 3020, Victoria, BC V8W 3N5, Telephone: (250) 721-7103; FAX: (250) 721-7120; E-mail:
[email protected]
Non-indigenous species represent potential threats to the persistence of native species. It is therefore important to understand the ecology of
introduced species in order to develop appropriate mitigation strategies when needed. Here, I describe some basic demographic attributes of
common wall lizards (Podarcis muralis), a European species, introduced near Victoria, British Columbia, in the early 1970s. Male and female wall
lizards reached similar body lengths, but males had relatively longer tails and were heavier than non-gravid females. Gravid females were found
in all months from May-July, inclusive, but hatchlings did not appear in the field before late August. Thus, individual females apparently produce
only one clutch per year. Growth rate was highest in small lizards and maturity probably was attained in the second full summer of life. As is
generally true of injury patterns, large lizards were more likely than small lizards to have experienced tail loss prior to capture. These results
suggest that P. muralis on southern Vancouver Island are fundamentally similar demographically to conspecifics at sites within the species'
natural range in Europe. However, whether P. muralis on Vancouver Island is a threat to the native northern alligator lizard, Elgaria coerulea,
remains an open question. Anecdotal observations suggest that wall lizards negatively affect alligator lizards, but both species coexist at some
sites and recent studies of behavioural interactions between the two species are somewhat equivocal.
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EXPLORING THE LIMITS OF A RANGE: Thamnophis sauritus IN NOVA SCOTIA
T. Herman1, R. Wassersug2, J. McNeil1, J. Todd 2, S. Bell2, G. Bourque1, B. Caverhill1, M. Lawton1, E. Newton1, N. Seguin1 and J. Caron1
1Biology Department, Acadia University, Wolfville NS B4P 2R6
2Department of Anatomy & Neurobiology, 5850 College Street, Dalhousie University, Halifax, NS B3H 1X5, 1902 585 1469 2902 494 2244 ph
1902 585 1059 fax
[email protected], [email protected], [email protected], [email protected], [email protected], [email protected],
[email protected], [email protected], [email protected], [email protected], [email protected]
In Canada, the Eastern Ribbonsnake (Thamnophis sauritus) reaches its northeastern limit in isolated pockets of southwestern Nova Scotia. We
used 1) historical records and extensive visual searching to locate populations in Nova Scotia, and 2) radio-telemetry, capture-mark-recapture
and direct observation to assess abundance, activity and movement, and to locate hibernacula at one intensively sampled site in Kejimkujik
National Park. At this site 105 individuals were marked in 2001, and a subset of 13 adults were implanted with radio transmitters and tracked
from May until November. At the same site in 2004, 135 snakes were observed and captured, and their positions, behaviour and environmental
characteristics noted. In April, newly emerged snakes were most abundant in two relatively rocky areas at the edge of a floodplain. By mid-May
all snakes were immediately adjacent to the nearest body of water. In September snakes concentrated in a grassy area several meters from
water. One presumed mating aggregation of 10+ snakes in <5 m radius was observed at this time. By mid-October most snakes were again in
rocky areas away from water. Snakes were rarely observed basking fully exposed. Based on 11 observations of feeding on anurans (Ranidae)
and fish (Cyprinidae), the species appears to be a sedentary ambush predator. Radio telemetry yielded only one hibernaculum, but spring and fall
concentrations suggest that snakes hibernate in rocky areas near the water table. Our extensive searches have confirmed the species' presence
on three watersheds in southwestern Nova Scotia. However, success of these labour-intensive efforts has been limited by the apparently patchy
distribution of the species in space and time, its cryptic nature and the high density of vegetative cover in its preferred habitats.
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Xenopus tropicalis: A NOVEL SURROGATE SPECIES FOR AMPHIBIAN TOXICOLOGY
Natacha S. Hogan and Vance L. Trudeau
Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ontario, Ph: (613) 562-5800
ext.6015, Fax: (613) 562-5486, [email protected] , [email protected]
With the development of standardized toxicity testing protocols, amphibians are becoming a favoured model animal for evaluating the effects of
environmental compounds. As with fish, individual amphibian species have unique characteristics that make them suitable for measuring specific
endpoints. Size of larvae and adults, developmental rate, animal husbandry and ease of rearing are some factors to consider when choosing a
species model. Xenopus laevis has long been exploited as a laboratory animal for studying early vertebrate development and more recently as a
surrogate species for amphibian toxicity testing. However, X. tropicalis also presents unique opportunities to study how aquatic pollutants affect
vertebrate development. X. tropicalis has a shorter generation time of 4-6 months and the adults are significantly smaller than X. laevis. In
addition to these rearing advantages, X. tropicalis has one of the smallest genomes among amphibians (20 chromosomes, half the size of X.
laevis) and is a diploid species. In fact, a genome sequencing initiative for X. tropicalis is underway and near completion (consult:
http://genome.jgi-psf.org/Xentr3/Xentr3.home.html). We have established a colony of X. tropicalis and are currently optimizing conditions for
tadpole rearing. In this presentation, we will describe some of our current work using X. tropicalis in comparative toxicity testing, along with future
projects involving the characterisation of sexual development and assessment of endocrine disruption using thyroid-response assays.
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THE GENETICS OF PERIPHERAL POPULATIONS
Briar J. Howes and Stephen C. Lougheed
Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6, Tel: (613) 533-6128, (613) 533-6000 ext. 75051, Fax: (613) 533-6617,
[email protected] , [email protected]
All species, no matter how endemic or broadly distributed, have ranges that are geographically circumscribed. Evolutionary hypotheses suggest
that a species' range is limited because geographically peripheral populations do not adequately adapt to their local conditions and continue
range expansion. It has been suggested that peripheral populations lack the genetic variation needed to adapt to different conditions beyond the
species' range, or that local adaptation in peripheral populations is precluded because of asymmetrical gene flow from more geographically
central populations. We test these hypotheses using genetic data (mtDNA and microsatellites) from 52 populations across the range of the five-lined skink (Eumeces fasciatus). Specifically, we examine how post-glacial dynamics and historical range fragmentation may have shaped
intraspecific diversity, whether "soft" peripheral populations (populations which are not bordered by an obvious physical boundary) and "hard"
peripheral populations (populations which are bordered by an obviously inhospitable boundary) display different patterns of intrapopulation
genetic diversity, and evaluate whether immigration significantly exceeds emigration in peripheral populations of this species. Our results shed
light on the genetic factors involved in the maintenance of a species range and help us to understand the forces acting on our own northern
peripheral populations of eastern Canada's only lizard species.
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THE EFFECT OF SEX RATIO ON SEXUAL SELECTION IN PAINTED TURTLES (Chrysemys picta) /L'EFFET DU RAPPORT DE SEXE SUR
LA SÉLECTION SEXUELLE CHEZ LES TORTUES PEINTES (Chrysemys picta)
Elinor J. Hughes1 and Ronald J. Brooks2
1Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Phone: 519-824-4120 x58360, Email: [email protected]
2Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Phone: 519-824-4120 x53944, Email: [email protected]
Sexual selection models predict that in systems based on female mate choice, the intensity of sexual selection on male traits will increase as the
sex ratio becomes more male-biased. I am investigating the influence of sex ratio on sexual selection in two populations of painted turtle
(Chrysemys picta): one female-biased and one male-biased. Painted turtle mating is based on female choice; male painted turtles are much
smaller than females (making coercion unlikely) and males court females by displaying with highly elongated foreclaws. Copulation is not limited
by gametogenesis in painted turtles and models indicate that variation in male mating success is small when there is little limitation on mating
opportunities. Hence, I hypothesize that sex ratio will not influence sexual selection in painted turtles. I will investigate three main questions. First,
are sexually dimorphic traits in male painted turtles maintained in the population by female mate choice and do these traits truly signal male
quality? Second, are there other factors that influence sexual selection in painted turtles? Third, is sexual selection influenced by the sex ratio of
the population? By answering these questions, I will be able to create a sexual selection model for pond turtles that could be used to further our
understanding of sexual trait divergence. Financed by an NSERC grant to R.J. Brooks.
Les modèles de sélection sexuelle prévoient que dans les systèmes où la femelle possède le choix d'accouplement, l'intensité de la sélection sur
les traits masculins augmentera comme le rapport de sexe devient plus biaisé vers les mâles. J'étudie l'influence du rapport de sexe sur la
sélection sexuelle dans deux populations de tortues peintes (Chrysemys picta) avec les rapport de sexe ci-contre. L'accouplement chez les
tortues peintes est basé sur le choix de la femelle. Les tortues peintes mâles sont plus petits que les femelles, rendant la coercition peu probable,
et les mâles courtisent les femelles avec leurs griffes de main élongées. La copulation n'est pas limitée par la gamétogenèse, et les modèles
indiquent que le succès reproductif chez les mâles possède peu de variation lorsque les opportunités d'accouplement ne sont pas limitées. Donc,
je suppose que le rapport de sexe n'influence pas la sélection sexuelle chez les tortues peintes. J'étudierai trois questions: si le dimorphism des
traits chez les tortues peintes mâles est maintenu dans la population par le choix d'accouplement des femelles et si ces traits signalent vraiment
la qualité masculine, s'il y a d'autres facteurs qui influencent la sélection sexuelle chez les tortues peintes et si la sélection sexuelle est influencée
par le rapport de sexe pour la population. Puis, je développerai un modèle de sélection sexuelle pour les tortues qui pourra enrichir notre
compréhension de la divergence des traits sexuels. Financé par une allocation de CRSNG à R.J. Brooks.
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LARGE BODY TEMPERATURE FLUCTUATIONS OF EASTERN FOXSNAKES (Elaphe gloydi) DURING VOLUNTARY COLD-WATER
SWIMMING IN THEIR NATURAL HABITATS
Anna Lawson1, Carrie A. MacKinnon2, E. D. Stevens3, Ronald J. Brooks4
Department of Integrative Biology, University of Guelph
1Telephone: 705 745-4458, [email protected]
2Telephone: 519 928-2837, [email protected]
3Telephone: 519 824-4120 ext. 52137 , [email protected]
4Telephone: 519 824-4120 ext. 53944, [email protected]
We examined thermal biology of free-ranging terrestrial foxsnakes (Elaphe gloydi), voluntarily swimming in cold water during spring, in Georgian
Bay, Canada. Using temperature-sensitive radio-telemetry, we recorded body temperatures of foxsnakes during 12 cold-water swims, and
subsequent warming on shore. During these swims, water temperatures ranged from 11-22�C and distances of 85-1330 m were travelled.
Snakes that were in cold water long enough equilibrated with water temperature and did not maintain a body temperature above ambient. The
largest observed drop in body temperature was 22.6�C (over 11 min) and the largest increase was 23�C (over 66 min). Such large, rapid
temperature fluctuations have not previously been reported in detail from snakes in the field. Twice as many telemetry observations as expected
occurred between 12:00 and 14:00, suggesting snakes chose to swim midday. Additionally, our results suggest that foxsnakes bask to raise their
temperature prior to swimming in cold water. We compared swimming speed and the coefficient of temperature change among foxsnakes and
other snake species. Swimming speed was positively correlated with water temperature.
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AMPHIBIAN CONSERVATION: BACK TO THE FUTURE
David Lesbarrères1 and Mike Fowler2
1Biology Department - Laurentian University, Ramsey Lake Road, Sudbury, Ontario, P3E 2C6, E-mail: [email protected] , Tel: 1-(705)
675-1151 x2275, Fax: 1-(705) 675-4859
2Integrative Ecology Unit, Department of Biological and Environmental Sciences, PO Box 65 (Biocenter 3, Viikinkaari 1), FIN-00014 University of
Helsinki, Finland. E-mail: [email protected] , Tel: +358-9191-57730
The success of many pond restorations is not well documented. Following a highway construction, a restoration begun in 1999 in western France
allowing the assessment of restoration efforts and changes through time. A survey was carried out on the amphibian community of eight ponds
before they were destroyed. Substitutive ponds were created according to precise floristic and pedological criteria, following the old pond
characteristics and taking into account the species attending them. Data are presented on species richness and ecological factors for the new
ponds and compared to the initial levels. Presence of amphibian species was recorded every year during the breeding period. Species richness
and abundance declined during the two years consecutive to the construction of the substitutive ponds but they increased thereafter, recovering
in most of the cases to the initial levels. Fauna and flora indexes were correlated until a threshold after which over vegetation had negative
effects on the establishment of amphibian populations. Likewise, an increase in the number of vegetation strata was positively correlated with
species richness. Population models were used, incorporating landscape characteristics to assess current and long term population viability. The
success of the restoration is specific and amphibian species present different colonisation capacities, improving our understanding of the
maintenance of species richness in aquatic environments.
Le succès des projets de restauration de mares est mal documenté. A la suite de la construction d'une autoroute, un projet de restauration a
débuté en 1999 dans l'ouest de la France permettant l'évaluation de ce type de projet ainsi que la dynamique des populations d'amphibiens
concernées. Une étude a été menée sur les communautés d'amphibiens de huit mares avant que celles-ci ne soient détruites. Des mares de
substitution ont été créées en suivant les caractéristiques écologiques des anciennes mares et des espèces observées jusqu'alors. La présence
des espèces a été mesurée chaque année lors de la période de reproduction. La richesse spécifique et l'abondance ont diminué durant les deux
années qui ont suivi la construction des nouvelles mares mais ces valeurs ont augmenté par la suite, retrouvant leurs valeurs initiales dans la
plupart des cas. Une corrélation a été observée entre des indices de faune et de flore jusqu'à un seuil au-delà duquel un surplus de végétation a
un effet négatif sur l'établissement des populations d'amphibiens. De la même manière, l'augmentation du nombre de strates végétales est
positivement associée avec la richesse spécifique. Des modèles populationnels incorporant les caractéristiques écologiques des mares ont
ensuite été utilisés pour estimer la viabilité présente et à long terme des populations. Nous avons montré que le succès du projet de restauration
est spécifique et que les espèces présentent différentes capacités de colonisation, améliorant notre compréhension du maintien de la richesse
spécifique en milieu aquatique.
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SURVIVORSHIP AND DIFFERENTIAL LONGEVITY IN THE SPOTTED TURTLE (Clemmys guttata)
Jacqueline D. Litzgus
Affiliation: Department of Biology, Laurentian University, Sudbury, ON P3E 2C6, Phone: 705-675-1151 ext 2314, Fax: 705-675-4859, Email:
[email protected]
Turtles are in decline world-wide, and few studies have collected the long-term, age-specific demographic data needed to construct life tables
and thus identify the life history stages critical to population viability and conservation. Here I report estimates of survivorship and longevity of the
spotted turtle (Clemmys guttata) using modified logarithmic decay equations and 24 years of mark-recapture data collected from a population at
the northern extreme of the species' range in Ontario. Spotted turtle survivorship and longevity estimates are among the highest values reported
for any animal species, and females are substantially more long-lived than males. Minimum annual adult female survivorship is 96.5%, maximum
longevity is 110 years, and age at maturity is 12 years. Minimum male survivorship is 94.2%, maximum longevity is 65 years, and age at maturity
is 11 years. The greater longevity of females may have evolved as a compensatory mechanism to offset delayed sexual maturity and low annual
clutch frequency in the north. The selective pressure on males for extended longevity may not be as great because their fitness can be ensured
in a shorter time frame through the species' promiscuous mating system. Alternatively, males may live shorter lives as a result of a greater cost of
reproduction (e.g., sperm production, courtship, copulation). This ongoing study is the longest-running on the spotted turtle, yet insufficient age-specific data have been gathered to construct a life table, particularly because egg and hatchling survival rates remain unknown; future work
should specifically focus on gathering such data. The results of the current study have important management implications when considering
which life history stages to protect for maintenance of population viability of long-lived vertebrates.
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PERIPHERAL POPULATIONS AND THEIR POTENTIAL CONSERVATION VALUE (Plenary address)
Stephen C. Lougheed
Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6., Tel: (613) 533-6128, Fax: (613) 533-6617 , [email protected]
Canada is depauperate in herpetofaunal diversity compared to most other countries. Moreover, for many of the approximately 86 Canadian
amphibian and reptile species, the bulk of their ranges occur in the continental USA. Thus, many herpetofaunal species in Canada (and almost
all that are of conservation concern) are represented by northern, geographically peripheral populations only. Peripheral populations may have
conservation value because they tend to be diverged genetically and phenotypically from core populations, and because they may represent
future sites of speciation (i.e. Mayr's peripatric model of speciation). As such it has been argued that peripheral populations should receive
conservation priority both because they are repositories of unique aspects of intraspecific diversity that may be important in changing
environmental circumstances and because they may generate future diversity. Comprehensive empirical support for these suggestions is lacking
especially for amphibians and reptiles. An additional complication is that we still do not know why range boundaries exist, and thus what
precludes peripheral populations from adapting to local environmental circumstances and continuing range expansion. In my talk I will briefly
quantify and summarize the degree of peripherality of Canadian herpetofauna, explore the notion that peripheral populations have
disproportionate conservation value, and present a suite of hypotheses that have been proposed (but seldom adequately tested) to explain range
limits. I will argue that Canadian conservation strategies for species at risk would derive great benefit from a coordinated research effort to
address these key knowledge deficits.
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WHY DID THE REPTILE CROSS THE ROAD? LANDSCAPE FACTORS ASSOCIATED WITH ROAD MORTALITY OF SNAKES AND
TURTLES IN THE SOUTH EASTERN GEORGIAN BAY AREA
C. A. MacKinnon1, L. A. Moore3, and R. J. Brooks2
Department of Integrative Biology, University of Guelph, Guelph, Ontario, N1G 2W1
1[email protected] , Tel: (519) 928-2837
2[email protected] , Tel: (519) 824-4120, Fax: (519) 767-1656
3Georgian Bay Islands National Park, Parks Canada, P.O. Box 786, Fort Simpson, NT, X0E 0N0, [email protected] , Tel: (867) 695-3028
Road mortality is believed to contribute to population declines in snakes and turtles, but there have been few attempts to quantify landscape
features associated with road mortality in these taxa. A secondary road near Georgian Bay, Ontario (45 52'N, 80 50'W) was surveyed daily by
automobile between April and October, 2003 and 2004. We measured landscape factors associated with roadkill of reptiles using multivariate
statistics and a geographic information system (GIS). A total of 340 road crossings were analyzed (269 snakes, 71 turtles; 91% dead on the
road), 44 of which involved species at risk in Canada. Snake crossings peaked during August, whereas turtle crossings peaked during June.
Multiple regressions were performed to assess the relative importance of the measured landscape factors using 1) total reptile, 2) snake and 3)
turtle crossing counts for buffered, equal-length road segments as output variables. The following landscape variables explained much (R2 =
0.354) of the distribution of reptiles killed along this road: distance from Georgian Bay, driveways, buildings, road intersections, adjacent
wetlands, and water crossings. Roadkill tended to be closer to Georgian Bay and further from driveways. Factors contributing to roadkill for
species at risk should be considered prior to planning roadway expansion.
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PHYLOGEOGRAPHY OF STREAM SALAMANDERS IN QUEBEC AND LABRADOR
Tricia M. Markle and David M. Green
Redpath Museum, McGill University, 859 Sherbrooke West, Montreal, Quebec, H3A 2K6, Canada, Phone: (514) 398-4086 ext. 3190 or 4088,
Fax: (514) 398-3185, Email: [email protected] , [email protected]
The Northern Two-lined, Eurycea bislineata, and the Northern Dusky, Desmognathus fuscus, are both small stream dwelling salamanders
commonly found throughout eastern North America. In Canada, E. bislineata has an extensive northern range whereas D. fuscus is restricted to
southern Quebec and a single site in the Niagara Gorge of Ontario. What determines the northern range limits of these salamanders is unknown.
However, if the St. Lawrence River acts as a barrier to gene flow, then isolated populations of E. bislineata on the north shore may have had the
opportunity to adapt to local conditions and expand their range accordingly. Desmognathus fuscus may not have had the same opportunity, and
may be restricted to the south if populations on the northern periphery of the range are swamped by genes adapted to more southern conditions.
Accordingly, phylogeographic patterns throughout the Canadian ranges of these salamanders were investigated in 58 populations of E.
bislineata, including new populations in northern Quebec and Labrador, and 11 populations of D. fuscus. The range of E. bislineata was found to
be far more extensive in northern Canada than previously recorded. Sequence variation in the mitochondrial cytochrome b gene of E. bislineata
populations revealed a genetically distinct group of four adjacent populations on the north shore of the St. Lawrence River. Indications are that an
isolated population near Trois-Rivieres underwent a genetic change and spread northward. Although molecular analysis did not reveal any
differences between Labrador and Quebec groups, morphometric analysis of individuals from all the populations revealed a distinct Labrador
morphotype. For D. fuscus populations, no genetic or morphometric differences were found.
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A VALUABLE TOOL TO ASSESS THE STRENGTH OF BIOLOGICAL HYPOTHESES IN HERPETOLOGY: AKAIKE'S INFORMATION
CRITERION (AIC)
Marc J. Mazerolle
USGS Patuxent Wildlife Research Center, Laurel, MD 20708-4017 USA, Phone: (301) 497-5636, Fax: (301) 497-5545, Email:
[email protected]
Herpetologists frequently use observational studies to explain a given pattern, such as the number of individuals in a habitat patch, with a large
number of explanatory (i.e., independent) variables. To elucidate such relationships, we have long relied on hypothesis testing to include or
exclude variables in regression models, although the conclusions often depend on the approach used (e.g., forward, backward, stepwise
selection). Akaike's Information Criterion (AIC) is remarkably superior in model selection (i.e., variable selection) than hypothesis-based
approaches. It it is simple to compute and easy to understand, but more importantly, for a given data set, it provides a measure of the strength of
evidence for each model that represents a plausible biological hypothesis relative to the entire set of models considered. Using this approach,
one can then compute a weighted average of the estimate and precision for any given variable of interest across all the models considered. This
procedure, termed model-averaging or multimodel inference, yields precise and robust estimates. In this paper, I illustrate how these techniques
can be used in the conservation of amphibians and reptiles. The AIC and measures derived from it is should be routinely adopted by
herpetologists, particularly those conducting field studies.
Les herpétologistes utilisent fréquemment des études d'observation sur le terrain dans le but d'expliquer des patrons, tels que le nombre
d'individus dans une parcelle d'habitat, à l'aide de plusieurs variables explicatives (indépendantes). Afin d'élucider ce genre de relations, nous
avons utilisé pendant longtemps les tests d'hypothèses afin d'inclure ou d'exclure des variables dans des modèles de régression, bien que les
conclusions dépendent souvent de l'approche utilisée (e.g., sélection ascendante, descendante, pas à pas). Le critère d'information d'Akaike
(AIC) est remarquablement supérieur aux tests d'hypothèses pour la sélection de modèles (i.e., sélection de variables). En plus d'être facile à
calculer et à comprendre, l'AIC procure une mesure du degré d'évidence de chaque modèle qui représente une hypothèse biologique parmi
l'ensemble des modèles considérés. En utilisant cette approche, on peut également calculer une moyenne pondérée de l'estimé et de la
précision d'une variable d'intérêt pour l'ensemble des modèles considérés. Cette procédure, nommée pondération de modèle ou inférence multi-modèle, donne des estimés précis et robustes. J'illustre comment ces techniques peuvent être utilisées dans les travaux de conservation des
amphibiens et des reptiles. Les herpétologistes, particulièrement ceux menant des études de terrain, devraient adopter l'AIC et les mesures qui
en découlent.
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IMPACTS OF ROW CROP AGRICULTURE ON SEXUAL DEVELOPMENT OF ANURANS
Tana V. McDaniel1, Pamela A. Martin1, Chris Marvin2, Mark McMaster2, Jim Sherry2
1Canadian Wildlife Service, Environment Canada, PO Box 5050, 867 Lakeshore Rd E, Burlington, ON, L7R 4A6, email:
[email protected], [email protected]
2National Water Research Institute, Environment Canada, PO Box 5050, 867 Lakeshore Rd E, Burlington, ON, L7R 4A6
Intensive row crop agriculture featuring corn and soybean production, is predominant in southwestern Ontario where the two crops account for
over 50% of the total acreage of crops grown. This form of agriculture relies heavily on pesticide and nutrient inputs for continued success under
conventional systems. The corn herbicides atrazine and metolachlor, are the two most heavily applied pesticides in Ontario and are routinely
detected in tributaries draining agricultural watersheds. Field and laboratory studies have indicated exposure to atrazine may disrupt normal
sexual development in male anurans, causing reductions in testosterone levels, laryngeal muscle diameter, and abnormal testes development.
Pesticide levels and water quality data was collected from 33 farm ponds and agricultural drains in southwestern Ontario, two agricultural
reference sites as well as four non-agricultural reference sites in August and September, 2003 and monthly from April through August in 2004.
Atrazine and metolachlor were detectable in most samples, exceeding 1 �g/L at some sites. Blood samples were obtained from leopard and
green frogs for analysis of sex hormones, and vitellogenin. Gonads were excised for histomorphological assessment. Analysis of frogs from late
summer 2003, revealed that circulating testosterone levels were negatively correlated with atrazine concentrations in pond water in juvenile male
leopard frogs (r = 0.74; p = 0.014) and adult female leopard frogs (r = 0.59; p < 0.008). No relationship was found between sex steroid levels in
frog plasma and concentrations of atrazine and metolachlor in the water in 2004, contrary to results from 2003. This may be due to lower
concentrations of atrazine and metolachlor in 2004. Agricultural sites, particularly those in Chatham exhibited a high percentage of males with
ova-testes (45%), as compared to control sites (10%).
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INFLUENCE OF WATER CONDITIONS ON THE EMBRYONIC SURVIVORSHIP OF THE OREGON SPOTTED FROG (Rana pretiosa)
René McKibbin1, Christine Bishop2, and Russ Haycock3
Canadian Wildlife Service, 5421 Robertson Rd, Delta, BC V4K 3N2.
1Tel 604-940 4728. Fax 604-946 7022. [email protected]
2Tel 604-940 4671. Fax 604-946 7022. [email protected]
3Hyla Environmental Services Ltd., 636 Thurston Close, Port Moody. BC, V3H 4J3, Tel 604-469 2910. Fax 604-469-5753. [email protected]
The Oregon spotted frog (Rana pretiosa) was listed as endangered in an emergency listing in 1999 by the Committee on the Status of
Endangered Wildlife in Canada [COSEWIC]. There are only three known locations of the subpopulation in Canada. These known subpopulations
are located in isolated sites in the extreme southwest corner of British Columbia. One of the populations has shown a steady decline since 1997.
At present, the cause of poor fecundity and embryonic survivorship at one of the sub sites at the Aldergrove site is not known but indicates the
need to obtain an understanding of embryonic survivorship in the context of population viability research. This research examined the question of
whether water conditions influence the embryonic survivorship at two subpopulations located at Seabird Island and Aldergrove, BC. A sub-sample of 15-30 eggs per clutch was transferred into Nytex holding cages and development monitored in-situ using Gosner staging tables. Free
swimming hatchlings were released at the site where the eggs were oviposited. Water conditions at each site were assessed by collecting water
samples for trace metal analysis, fecal and total coliform and water chemistry. Water temperature was also recorded. At Aldergrove embryonic
survivorship varied between 0.09 and 0.45 at sub site A and 0.71 and 0.96 at sub site B. At Seabird Island embryonic survivorship was between
0.67 and 0.86. Initial analyses indicate that no extreme water quality conditions occur at any of the two study sites and most likely do not
contribute to poor embryonic survivorship at Aldergrove.
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UPDATE FROM THE KAWARTHA TURTLE TRAUMA CENTRE
Kristy McNab
Kawartha Turtle Trauma Centre, c/o Riverview Park & Zoo, PO Box 4125 Peterborough ON. K9J 6Z5, Phone: (705) 748-9301 ext. 2303, email:
[email protected]
The Kawartha Turtle Trauma Centre in Peterborough, Ontario, has been treating road injured turtles for four seasons and is receiving turtles from
across the province, often from other wildlife rehabilitators. Painted, Snapping and Blanding's turtles are the three species most often admitted
to the centre. Snapping turtles have a high incidence of skull and jaw fractures and carapace shearing, and Painted turtles are usually admitted
for shell fractures. Blanding's turtles are often treated for carapace, bridge and jaw fractures. Of great concern is the prevalence of eye injury in
Blanding's turtles; severe visual impairment has prevented the release of several Blanding's turtles. The method of fracture fixation most
commonly used is orthopedic wire. Oesophagostomy feeding tubes are often placed and may be used for months before patients begin to eat on
their own. Antibiotic therapy is being refined and the use of pain medication is increasing, the standard being that every patient recieves at least
short term analgesia. Field triage and first aid may increase treatment success and release. Most important in first aid is to keep the injured
animal hydrated either through soaking or the use of subcutaneous fluids. Heat stress can be treated by placing wet towels or gauze on the
turtle. Mobile shell fractures can be stabilized with tape. For Blanding's turtles we encourage the use of tear gel or wet gauze lightly draped over
the head to prevent further eye injury through dessication. The Centre has received its third cohort of Wood turtles to headstart. Wood turtles are
being raised at the Centre for release into an endangerd population. Weights of 250g or more were reached after 20 months at the Centre.
Individuals attaining sufficient size will be equipped with transmitters and released. We hope that the expertise developed in captive rearing will
be potentially used for other captive rearing initiatives.
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GENETIC STRUCTURE OF THE EASTERN RED-BACKED SALAMANDER (Plethodon cinereus) IN AN URBAN LANDSCAPE
Sarah Noël1, Martin Ouellet2, Patrick Galois2 and François-Joseph Lapointe1
1Dép. sciences biologiques, Université de Montréal, C.P. 6128 Succ. Centre-Ville, Montréal, Qc, H3C 3J7, [email protected] , [email protected]
2Amphibia-Nature, 4254 rue Garnier, Montréal, Qc, H2J 3R5, Tel: 514-343-6111 ext.1031, Fax:514-343-2293, [email protected] ,
[email protected]
The Mont Royal, located in the heart of Montréal, represents an important reservoir of biodiversity in the urban landscape. However, it has been
gradually fragmented by anthropogenic activities since the arrival of the first European settlers more than 350 years ago. Nowadays the Mont
Royal presents a mosaic of forested patches isolated by roads, graveyards and buildings, into which four Eastern Red-backed Salamander
(Plethodon cinereus) populations are still found. To evaluate the effect of this habitat fragmentation, genetic diversity and population
differentiation were assessed with microsatellite loci and compared to the genetic structure of four populations situated in a continuous habitat,
the Mont Mégantic. Results indicate that allelic richness and heterozygosity are lower in the fragmented populations. Exact differentiation tests
and pairwise FST showed that the four populations from Mont Royal could be differentiated, whether populations from Mont Mégantic were non-differentiated. These results indicate that urbanization of the Mont Royal has significantly changed the genetic structure of the Eastern Red-backed Salamander and raise conservation concerns for these isolated populations.
Le Mont Royal, situé au coeur de Montréal, constitue un site important pour la biodiversité dans le paysage urbain. Toutefois, cette montagne a
été graduellement fragmentée par les activités anthropiques depuis l'arrivée des premiers colons il y a plus de 350 ans. Aujourd'hui, le Mont
Royal présente une mosaïque d'îlots forestiers isolés par des édifices, des cimetières, et des routes, parmi lesquels on retrouve quatre
populations de salamandre cendrée (Plethodon cinereus). Afin d'évaluer les effets de cette fragmentation d'habitat, la diversité génétique et la
différentiation des populations ont été évaluées à l'aide de marqueurs microsatelllites et comparées à la structure génétique de quatre
populations situées dans un habitat non-fragmenté, le Mont Mégantic. Les résultats indiquent que la richesse allélique et l'hétérozygotie sont plus
faibles pour les populations fragmentées. Les tests exacts de différentiation et les valeurs de Fst montrent que les quatre populations du Mont
Royal sont différentes les unes des autres alors que les populations du Mont Mégantic sont similaires entre elles. Ces résultats indiquent
clairement que la fragmentation du Mont Royal a modifié la structure génétique des populations de salamandres cendrées et sont inquiétants
pour la survie de ces populations isolées.
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THE DRASTIC DECLINE OF THE WESTERN CHORUS FROG (Pseudacris triseriata) IN SOUTHWESTERN QUÉBEC
Isabelle Picard and Jean-François Desroches
1050-1 Saint-Marc, Sherbrooke (Québec), J1K 2T7 Canada, [email protected]
The Western Chorus Frog (Pseudacris triseriata) has undergone a drastic decline in southwestern Québec over the past 50 years; the species is
now absent from about 90 % of its historical range. In April 2004, an exhaustive survey of Chorus Frog populations was made throughout its
present range, with auditory monitoring at breeding ponds, in order to locate these ponds precisely and evaluate more precisely the status of this
frog. A total of 803 breeding ponds dispersed in 9 metapopulations and 7 isolated populations were located. Most of these are highly at risk of
destruction by residential developments. The Western Chorus Frog was not found at 4 sites where it occurred recently. Declines are observed in
5 of the 9 present metapopulations. A second survey of the breeding ponds later in the summer showed that at least 5 % of them were
destroyed, and it up to 25 % in some areas. Despite the fact that the species was officially designated 'vulnerable' in Québec in 2000, the
destruction of its habitats continues and at this rate the Western Chorus Frog may be extirpated from southwestern Québec in 10 to 25 years.
La Rainette faux-grillon de l'Ouest (Pseudacris triseriata) a subi un important déclin dans le sud-ouest du Québec depuis 50 ans; l'espèce est
maintenant absente dans environ 90 % de son aire historique. En avril 2004, un inventaire exhaustif des populations de Rainettes faux-grillon a
été réalisé dans son aire actuelle, à l'aide de l'écoute des chants, dans le but de localiser précisément les étangs de reproduction et d'évaluer de
façon plus précise le statut de cette rainette. Au total, 803 étangs de reproduction répartis dans 9 métapopulations et 7 populations isolées ont
été recensés, dont la plupart sont menacées par le développement résidentiel. La Rainette faux-grillon de l'Ouest est disparue de 4 sites où elle
était présente récemment. Des déclins ont été observés dans 5 des 9 métapopulations actuelles. Un deuxième inventaire des étangs de
reproduction réalisé plus tard dans l'été 2004 a démontré que pas moins de 5 % de ceux-ci avaient été détruits, et ce chiffre atteint 25 % dans
certains secteurs. Malgré le fait que l'espèce ait été officiellement désignée 'vulnérable' au Québec en 2000, la destruction de ses habitats
continue et à ce rythme la Rainette faux-grillon de l'Ouest pourrait disparaître du sud-ouest du Québec dans 10 à 25 ans.
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HOW FAR FROM THE NESTING SITE SHOULD WE PROTECT THE FOUR-TOED SALAMANDER (Hemidactylium scutatum)? A CASE OF
URBAN CONSERVATION
Daniel Pouliot1 and Héloïse Bastien2
14230 #1 Louis-Lacroix, Trois-Rivières, Québec. G8Y 5V2 819-697-2405 [email protected]
2Héloïse Bastien : Direction de l'aménagement de la faune de la Capitale-Nationale, Ministère des Ressources naturelles et de la Faune 365, 55e
Rue Ouest, Charlesbourg (Québec) G1H 7M7 Téléphone : (418) 644-8844 poste 305, [email protected]
Shortly after the discovery in 1999, of a four-toed salamander (Hemidactylium scutatum) population in the periphery of Quebec City, the
modification and destruction of the forest surrounding the nesting site began. To minimize the damages to this rare species population by the
residential development, we investigated the distance from the nesting site, in the terrestrial habitat, use by the four-toed salamander outside the
nesting time. Our results proposed that the four-toed salamander doesn't go farther than 50 meters from the nesting site. The "Centre de
données sur le patrimoine naturel du Québec" proposed a buffer zone of 150 meters. We feature that the 150 meters buffer zone should be view
as two areas. Any activities or development, neither hiking trail, should be planned in the first 50m from the nesting site. Only hiking trail could be
managed in the 50 to 150 meters from the nesting site. Moreover, special attention should be done to possible hydrologic modifications
consequent of habitat modification outside the 150 meters buffer zone.
Peu après la découverte en 1999, d'une population de salamandres à quatre orteils (Hemidactylium scutatum) dans la banlieue de la ville de
Québec, la modification et la destruction de l'habitat forestier entourant le site de ponte débuta. De manière à minimiser l'impact du
développement résidentiel, nous avons cherché à quantifier la distance du site de ponte, dans l'habitat terrestre, utilisée par la salamandre à
quatre orteils, en-dehors de la période de ponte. Nos résultats suggèrent que la salamandre à quatre orteils ne s'éloignent pas à plus de 50
mètres du site de ponte. Le Centre de données sur le patrimoine naturel du Québec propose une zone tampon de 150 mètres. Nous soulignons
que cette zone devrait-être considérée comme deux aires distinctes. Aucune activités ou dévelopment, pas même des sentiers de randonnée
pédestre, ne devrait être prévues dans les premiers 50 mètres du site de ponte. Uniquement des sentiers pédestres devrait-être tolérés dans la
zone de 50 à 150 mètres du site de ponte. De plus, une attention toute particulière doit-être portée à d'éventuelles modifications des conditions
hydrologiques du milieu, engendrés par des travaux réalisés au delà de la zone tampon de 150 mètres.
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EFFECT OF TEMPERATURE ON PHYSIOLOGY AND BEHAVIOUR IN TWO COLOUR MORPHS OF THE RED-BACKED SALAMANDER
(Plethodon cinereus)
Daniel J. Reeves1,2, Jacqueline D. Litzgus2, and David Hackett1
1Department of Biology, Nipissing University, North Bay, ON P1B 8L7
2Department of Biology, Laurentian University, Sudbury, ON P3E 2C6
Phone: 705-675-1151 ext 2314, Fax: 705-675-4859, Email: [email protected]
Colour polymorphisms are common in amphibians and are often viewed as adaptations to specific ecological conditions. The terrestrial eastern
red-backed salamander (Plethodon cinereus) is widely distributed in forests of eastern North America, and displays colour polymorphism.
Populations usually exhibit two colour morphs - a red-backed morph and a lead-backed morph, and the proportion of each morph in a population
is correlated with climate. The purpose of the current study was to investigate the effect of temperature on physiological (metabolic rate) and
behavioural differences between the two morphs. We hypothesised that: 1) temperature affects metabolic rate differently between the two
morphs; 2) the two morphs differ in agonistic behaviour when paired against con-specifics; and 3) temperature affects the degree of agonistic
behaviour. With respect to the first hypothesis, we found a trend for a difference in metabolic rate between the morphs; however, the difference
was not statistically significantly at our sample size. The second and third hypotheses were supported. In agonistic trials at the warmer
temperature (20�C), resident red-backed morphs exhibited more aggressive behaviours and fewer submissive behaviours than lead-backed
intruders. In contrast, resident lead-back morphs were not more aggressive than red-backed intruders. At the cooler temperature (9�C), the
proportion of aggressive and submissive behaviours did not differ between the morphs regardless of who was the resident. In general,
salamanders exhibited more aggressive behaviours at higher temperatures than at lower temperatures. We discuss our findings in light of the
species' distribution and frequencies of each colour morph in wild populations.
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ANNUAL TEMPERATURE VARIATIONS AFFECT CLUTCH FREQUENCY AND EGG SIZE IN A NORTHERN POPULATION OF PAINTED
TURTLES (Chrysemys picta)
Njal Rollinson and Ron Brooks
Department of Integrative Biology, University of Guelph, ON, N1G 2W1, 519-824-4120 ext. 58360, [email protected] , [email protected]
Annual variations in clutch frequency (CF) and mean egg size (ES) have been clearly documented in several populations of Chrysemys picta.
Although it has been suggested that these annual differences are temperature-mediated, previous efforts to relate variations in CF and ES to
temperature have been largely unsuccessful. We investigated the relationship between annual temperature variation and CF and ES over 12
years in Algonquin Provincial Park. We predicted that warmer temperatures during the summer and fall prior to nesting and during the following
spring would be associated with an increase in CF and a decrease in egg mass (EM) and egg length (EL). EM and EL were not related to fall or
spring temperatures; however, an a-posteriori analysis revealed that both these parameters were negatively related to temperatures just prior to
nesting season onset (r2 = 0.60-0.75, 9-13 years analyzed). We further investigated the effect of individual thermal regimes on ES by attaching
Thermochron iButtons to the shells of ~50 females over two field seasons. Preliminary analyses appear to support the idea that temperature and
ES are inversely related. We argue that, if temperature acts as a constraint on ES, then these findings support optimal egg size theory.
Preliminary analyses further indicate that a strong relationship exists between the temperature of the previous summer and CF.
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WOOD TURTLE (Clemmys insculpta) HABITAT REQUIREMENTS AND MOVEMENTS IN NEW BRUNSWICK
Vanessa Roy and Graham Forbes
University of New Brunswick, Faculty of Forestry and Environmental Management, P.O. Box 44555, 28 Dineen Drive, University of New
Brunswick, Fredericton, NB E3B 6C2, Tel: 506-452-6315, Email: [email protected] , [email protected]
In Canada, the Wood Turtle is listed as a species of concern by COSEWIC. Efforts are being undertaken to protect the remaining populations
within the country. A two-year habitat selection study of Wood Turtles was undertaken by UNB from May to August 2003-2004, within the military
training area of CFB Gagetown in New Brunswick. A limited number of studies have quantitatively measured habitat selection in Wood Turtles in
the northern extent of their range. These studies suggest that proximity to water, low canopy cover and non-forested locations such as alder
stands are selected habitat features. We hypothesize that male habitat selection is due largely to their search for females and competition with
males. Two person visual searches of selected waterways along with radio-telemetry of 30 turtles were conducted over two seasons. Habitat
characteristics were measured at each capture site and a corresponding random site. Conditional Logistic Regression was used to determine
habitat selection models. A total of 115 individual turtles were captured over two seasons. Although non-forested locations were favoured overall,
forested locations did see a significant increase in use later in the season. Distance to water varied throughout the season. The majority of
observations of Wood Turtles were noted within 300 m, several individuals were also noted at further distances than recorded in previous
studies. Habitat use was highly variable between individuals and throughout the active season. An initial observation of the data does not
suggest a difference between the sexes.
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FACTORS AFFECTING AMPHIBIAN DISTRIBUTION AND COMMUNITY STRUCTURE IN NOVA SCOTIA
Ronald W. Russell
Department of Biology, Saint Mary's University, 923 Robie St., Halifax, Nova Scotia, B3H 3C3, Phone: (902) 420-5645, Fax: (902) 496-8285,
Email: [email protected]
Nova Scotia wetlands were surveyed for amphibians from 2002 to 2005. Wetlands on game reserves, parks, coastal barrens, and developed
areas were included. Species richness in these wetlands was stable over the four study years, however there was substantial species turnover
between years. Water chemistry was generally not useful in predicting amphibian species richness. Acidic wetlands with pH less than 4 were not
used by amphibians. Wetland hydroperiod and presence of fish predators were major factors affecting species richness. Additionally, distance
from human disturbance was a significant factor in determining amphibian species richness in ponds. Community structure was assessed using
stable isotope ratios of N and C. Preliminary data indicate potential competitive relationships between wood frog and spring peeper tadpoles and
identified predatory invertebrates in amphibian communities.
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EXCAVATION OF FRESHWATER TURTLE EGGS IS A NON-DELETERIOUS METHOD FOR OBTAINING FECUNDITY AND EGG
MORPHOMETRIC DATA
Jason Samson1, Elinor J. Hughes, and Ronald J. Brooks2
1Department of Natural Resource Sciences. Macdonald Campus of McGill University., 21,111 Lakeshore Road, Sainte-Anne de Bellevue,
Québec, Canada. H9X-3V9, email:[email protected]
2Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada., N1E 2W7 519-824-4120 x58360, Email:
[email protected] , [email protected]
It has been suggested, and verified in artificial conditions, that handling eggs of various turtle species can be deleterious to embryos. However,
excavating turtle nests remains an ideal method for obtaining accurate fecundity and egg morphometric data. We tested the hypothesis that
handling turtle eggs decreases embryo survival in a population of midland painted turtle (Chrysemys picta marginata) that has been extensively
studied since 1978 and where more than 2300 nests have been excavated since 1983. We compared embryo survival in handled and non-handled natural nests during the summers of 2002, 2003 and 2004. All nests were protected from mammalian predators. Upon excavation of the
nests in the following springs, we did not find differences in survival between the two treatments, suggesting that the benefits in knowledge
gained from nest excavation far outweigh the possibility of a small increase in mortality that could arise from handling the eggs. Moreover, we
propose that excavating nests is beneficial for the management of endangered turtle species because the eggs can subsequently be relocated,
protected, or incubated for headstarting programs when needed.
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EMERGENT INFECTIOUS DISEASES IN THE LEOPARD FROG (Rana pipiens) IN CENTRAL ONTARIO
Valerie St-Amour1 and Michael Berrill2
1Department of Biology, Laurentian University, Sudbury, Ontario, Canada P3E 2C6, Email: [email protected] , Tel: 1-(705) 675-1151, Fax:
1-(705) 675-4859
2Department of Biology, Trent University, 1600 West Bank Drive, Peterborough, Ontario, Canada K9J 7B8, Email: [email protected] , Tel: 1-(705) 748-1011
Emergent infectious diseases (EID's) are one of the most pressing issues of amphibian conservation. They have been linked to several
amphibian declines and are continually being discovered in new areas and species. Two of the most serious diseases, Ranavirus and chytrid
fungus have recently been found in Ontario. Ranavirus was found both in woodfrog (Rana sylvatica) and leopard frog (R. pipiens) populations
while chytrid was present in the bullfrog (R. catesbeiana). Leopard frogs are currently sold and distributed across Ontario as bait and may be
contributing to the spread of EID's. Using genetic analysis and histological methods, wild, captive and bait shop samples of R.. pipiens were
analyzed to test the hypothesis that Ranavirus and chytrid were present in these Ontario populations. Local bait shops were sampled to
determine if infected frogs are being transported around Ontario through the recreational sale of bait frogs. Wild samples were also used to gain
a better idea of the range of the virus in central Ontario. Captive populations were kept to determine if the virus spread in captive situations due
to the immune suppression caused by stress. Chytrid was searched for in the wild samples to determine if it is playing a part in amphibian
declines in this region. Ranavirus was discovered in one of the six wild populations, in the bait shops and in all of the captive populations. Chytrid
was also present in several of the samples. For this reason the commercial harvesting of bait frogs should be stopped immediately.
Les maladies infectieuses émergentes sont un des plus importants problèmes dans la conservation des amphibiens. Elles sont responsables du
déclin de nombreuses populations et sont continuellement observées chez de nouvelles espèces. Deux de ces maladies, Ranavirus et le
champignon chytride ont récemment été découvertes en Ontario. Ranavirus a été observé chez la grenouille des bois (Rana sylvatica) et la
grenouille léopard du nord (R. pipiens) alors que le chytride fut observé chez le ouaouaron (R. catesbeiana). La grenouille léopard est vendue et
distribuée à travers l'Ontario en tant qu'appât et potentiellement contribue à la diffusion de ces maladies. Grâce à des analyses génétiques et
histologiques, des grenouilles léopard ont été testées pour ces deux maladies. Des magasins de pêche ont été échantillonnés pour déterminer si
des grenouilles infectées traversent l'Ontario au travers de l'activité de pêche à l'appât. L'étude de populations sauvages a permis de déterminer
l'expansion du virus dans le centre de la province. Enfin, des individus tenus en captivité ont également été analysés pour identifier une possible
diffusion du virus due à l'altération des défenses immunitaires en situation de stress. Le chytride a été recherche dans les populations sauvages
pour évaluer son rôle dans le déclin des amphibiens dans la région. Ranavirus a été découvert dans une des six populations sauvages
analysées, dans les magasins de pêche et dans toutes les populations captives. Le chytride était présent dans de nombreux échantillons. Pour
cette raison, la distribution commerciale de grenouille en tant qu'appât devrait cesser immédiatement.
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BEAVER (Castor canadensis) AS A SURROGATE SPECIES FOR CONSERVING ANURAN AMPHIBIANS ON BOREAL STREAMS
Cameron E. Stevens1, Cynthia A. Paszkowski2, and A. Lee Foote3
Dept. of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, 1 [email protected] ; P(780) 492-5751; F(780)492-9234, 2
[email protected],
3Dept. of Renewable Resources, University of Alberta, Edmonton, AB T6G 2H1; [email protected]
We explored the use of beaver (Castor canadensis) as a surrogate species for amphibian conservation on small (1st-4th order) streams in the
Boreal Foothills of west-central Alberta. First, we examined if the presence of beaver affected the abundance of anurans amphibians using call
surveys and pitfall trapping on unobstructed streams and beaver ponds. We also characterized the distribution of beaver ponds and the potential
effects of landscape features, road construction and forest harvesting on beaver pond occupancy of streams using a novel combination of a
digital elevation model and vegetation inventory data in GIS. Call surveys clearly suggested that beaver create breeding habitat for the boreal
chorus frog (Pseudacris maculata), wood frog (Rana sylvatica) and western toad (Bufo boreas). No calling males of any species were recorded
on unobstructed streams. Pitfall trapping showed that the wood frog exhibit high rates of juvenile recruitment on beaver ponds given that more
individuals were captured on beaver ponds versus unobstructed streams and that young-of year represented the majority of wood frog captures
(84%). A strong correlation between percent landscape occupied with beaver ponds and abundance of young-of-year wood frogs on
unobstructed stream suggests that anurans captured along streams originated from beaver ponds. Using a generalized linear mixed-effect
model, we found that the probability of beaver pond occurrence on streams was positively associated with elevation and stream order but
unrelated to riparian forest height and distance to nearest road. In addition, proximity of streams to cutblocks reduced the probability of beaver
pond occupancy even if Populus spp. was regenerating in the logged area. The high number and area of beaver ponds relative to basin (i.e.,
non-beaver) ponds in the Boreal Foothills suggests that beaver can play a key role in maintaining regional populations of amphibians. Beaver
food requirements and dam-building patterns should be incorporated in management strategies as part of a surrogate species approach to
amphibian conservation.
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STATUS OF THE WESTERN TOAD AND ITS USE OF 'BORROW PITS' IN THE FOOTHILLS OF WEST-CENTRAL ALBERTA
Cameron E. Stevens1, Cynthia A. Paszkowski2, David Stringer4 and Shelly Boss3
Dept. of Biological Sciences, University of Alberta, Edmonton, AB T6E 2E9, 1 [email protected]; P(780)492-5751; F(780)492-9234, 2
[email protected], 3 [email protected]
4#30 3705 Fonda Way SE, Calgary, AB T2A 6G9; [email protected]
Populations of western toad (Bufo boreas) have declined or disappeared from much of the species southern range in the U.S. To assess
distributions and densities of the western toad in the foothills of west-central Alberta, Canada we conducted visual surveys of 130 natural
waterbodies in watersheds of the Pembina and North Saskatchewan Rivers in early and late summer 2000. Encounter rates were consistently
low throughout the study area (0.2-0.3 toads/hr), one-tenth the rate for the co-occurring wood frog (Rana sylvatica), and nearly one-third the
value reported in the literature for a toad population in south-western British Columbia during the late 1970s. Large-scale pitfall trapping of
breeding and non-breeding sites during 2001 and 2002 showed differing age structures between wood frog and western toad populations, and
that recruitment of age-0 juveniles in western toad populations was low. On potential breeding ponds, more age-1+ toads were captured than
age-0 toads, and the difference in numbers between age classes was greater on 'borrow pits' (human-created roadside ponds) compared to
beaver ponds indicating that borrow pits might be ecological sinks providing poor larval habitat. Borrow pits had either dried prior to juvenile
emergence or had oligotrophic to mesotrophic waters compared to eutrophic states in beaver ponds. Pond creation as a management strategy or
product of road construction may put western toad populations at risk of decline in the foothills of Alberta.
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DOES MULTIPLE PATERNITY INCREASE WITH FEMALE SIZE IN THE COMMON MAP TURTLE (Graptemys geographica)?: A WORK IN
PROGRESS
Carine Verly1, Gregory Bulté2, Gabriel Blouin-Demers3
1 Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, ON, Canada, K1N 6N5. Telephone: (613) 562-5800-2574. Fax: (613)
562-5486. E-mail address: [email protected]
2Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, ON, Canada, K1N 6N5. Telephone: (613) 562-5800-2574. Fax: (613)
562-5486. E-mail address: [email protected]
3Department of Biology, University of Ottawa, 150 Louis Pasteur, Ottawa, ON, Canada, K1N 6N5. Telephone: (613) 562-5800-6749. Fax: (613)
562-5486. E-mail address: [email protected]
In general, larger females can produce more and/or bigger offspring. It has also been shown that larger offspring survive better. Therefore, larger
female map turtles should be more attractive to males. If males incur non-trivial costs of mating, such as missed opportunities or sperm limitation,
they should mate preferentially with large females. Accordingly, multiple paternity should be more common in larger females. To test this
prediction, we captured 34 gravid females spanning the full size range of reproductive females and induced egg laying with oxytocin. We then
collected blood samples from > 250 hatchlings for paternity analyses (the number of sires will be deducted from the paternal alleles at 5
microsatellite loci). Results will be compared to other turtle species for which multiple paternity has been assessed, in light of their mating
systems.
En général, les plus grosses femelles produisent plus et/ou de plus gros rejetons. Il a également été démontré que les plus gros rejetons ont une
meilleure chance de survie. Pour ces raisons, les mâles Tortues Géographiques devraient être plus attirés par les plus grosses femelles. Si les
mâles encourent des coûts de reproduction élevés, tels des pertes d'opportunités d'accouplement ou une limitation dans la spermatogénèse, ils
devraient s'accoupler préférentiellement avec de plus grosses femelles. Par consequent, la paternité multiple devrait être observée plus
fréquement dans les couvées pondues par des grosses femelles. Nous avons capturés 34 femelles gravides pour tester cette prédiction. La
ponte à été induite en laboratoire à l'aide d'oxytocine et nous avons prélevé des échantillons sanguins sur plus de 250 rejetons pour effectuer
des analyses de paternité (le nombres d'allèles présents à 5 loci de microsatellites permettront de déduire le nombre de pères par couvée). Les
résultats seront comparés aux autres espèces de tortues chez lesquelles des études de paternités multiples ont été effectuées et ce en lumière
de leur système de reproduction.
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LOCAL AND REGIONAL SCALE HABITAT SELECTION BY WOOD TURTLES (Glyptemys insculpta) AT THE NORTHERN LIMIT OF THEIR
RANGE / SÉLECTION D`HABITATS PAR LA TORTUE DES BOIS (Glyptemys insculpta) SUR L`ÉCHELLE LOCALE ET RÉGIONALE AU
NORD DE SON AIRE DE RÉPARTITION
Pamela Wesley1 and Dr. Ronald J. Brooks2
1Department of Integrative Biology University of Guelph, Guelph, ON, N1G 2W1, Tel: (519) 824-4120 x.58360, Fax: (519) 767-1656, Email:
[email protected]
2Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Tel: (519) 824-4120 x.53944, Fax: (519) 767-1656, Email:
[email protected]
Wood turtles (Glyptemys insculpta) are declining throughout their range, largely as a result of habitat loss. Previous research has focused
predominantly on studying movement patterns and habitat use/selection by wood turtles on the "local" scale. This study aims to identify "local"
and "regional" scale habitat features selected by wood turtles at the northern limit of their range, as well as to determine the most relevant
scale(s) of consideration with respect to resource management for wood turtle conservation. Local scale habitat selection by individual turtles
during their active season is investigated by means of radio-telemetry. Regional scale habitat selection by wood turtle populations is studied
through surveys of inhabited and uninhabited stream reaches. Logistic regression and Akaike's Information Criterion (AIC) will be used to
develop and evaluate a number of potential habitat selection models at both scales. Beyond identifying environmental features significant to the
wood turtle, successful models resulting from the landscape scale study may also be used to map potential wood turtle habitat that has not yet
been identified.
La tortue des bois (Glyptemys insculpta) décline à travers son aire de répartition, en grande partie dû à la perte de son habitat. Jusqu'à présent,
la recherche s'est concentrée sur les patrons de mouvement et sur l'utilisation ou la sélection d'habitats sur l'échelle locale. Cette étude a pour
but d'identifier les caractéristiques d'habitat choisies par la tortue des bois sur l'échelle locale ainsi que sur l'échelle régionale. De plus, cette
étude vise à déterminer la/les échelle(s) pertinente(s) à la gestion des ressources par rapport à la conservation de la tortue des bois. La sélection
d'habitats par des individus à l'échelle locale a été étudié par moyens de radio-télémétrie, tandis que la sélection d`habitats à l'échelle régionale a
été étudié par moyens de recherches faites sur des cours d'eau habités et inhabités par des populations de tortues des bois. La régression
logistique et �Akaike`s Information Criterion� seront utilisés afin de développer et d'évaluer plusieurs models sur chaque échelle. En plus de
pouvoir identifier les caractéristiques d'habitat de valeur à la tortue des bois, les models pourront être utilisés afin d'identifier de nouvelles régions
(non connues) composées d`habitat convenable à cette espèce.
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DOES FOREST HARVESTING CREATE OR DESTROY AQUATIC AMPHIBIAN HABITAT?
Elke Wind
E. Wind Consulting. 348 Machleary St., Nanaimo, BC V9R 2G9 , 250-716-1119, [email protected]
Amphibians utilize a variety of lentic habitats for breeding, including small, seasonal ponds. However, current legislation in British Columbia does
not provide protective measures for small wetland habitats less than 0.25 to 1 ha, depending on their location within the province. Island
Timberlands (formerly Weyerhaeuser) utilizes variable retention harvesting methods so that small tree patches are often retained around small,
ephemeral ponds. As part of the Forest Project, we initiated a pre- and post-harvest study in 2004 to investigate the effectiveness of retaining
buffers around small ponds at three forest sites containing 70+ ponds. The three treatments included: normal buffer width (1 tree width), double
buffer width ( 2 tree widths), and unbuffered. At all ponds, the hydroperiod, drying rate, and amphibian species presence and larval development
were recorded regularly. In Year One (2004), all sites and ponds were unharvested. In Year Two (2005), one site was harvested using patch
retention methods and two remained forested but roads were put in. In Year Three (2006), the remaining two sites will be also be harvested. The
ponds were highly variable in their water depths, hydroperiods, vegetative cover, searchability, and presence of amphibian species. In 2004, all
ponds dried by late July as it was a dry spring and summer. In contrast, spring 2005 was wet and the ponds retained water until late August,
especially those in the harvested site where water levels rose significantly. All three amphibian species returned to breed in ponds at the
harvested site in 2005 (Long-toed salamander, Red-legged frog, and Pacific Chorus frog). Evidence suggests that Pacific Chorus frogs may be
attracted to more open canopy ponds, potentially using roads as dispersal corridors, as they were common throughout the harvested site in 2005
and frequently observed breeding at ponds near roads in the two unharvested sites in 2005. Metamorphosis was confirmed at more sites in 2005
than in 2004.